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目的：复制高血糖症动物模型，研究高糖对大鼠血管内皮舒张功能的影响及其机制。方法：采用血管功能检测、组织培养及放射免疫分析方法。结果：①高血糖组血管乙酰胆硷依赖性血管舒张反应明显降低（Ｐ＜００１）；②高血糖组血浆ＮＯ－２含量、主动脉一氧化氮合成酶（ＮＯＳ）活性及环磷酸鸟苷（ｃＧＭＰ）含量明显低于对照组（Ｐ＜００５，Ｐ＜００１）。结论：长期高血糖症可引起血管内皮源性血管舒张因子／一氧化氮（ＥＤＲＦ／ＮＯ）系统出现严重障碍，从而降低了血管舒张功能
OBJECTIVE: To replicate the animal model of hyperglycemia and investigate the effect of high glucose on the diastolic function of vascular endothelial cells in rats and its mechanism. Methods: Vascular function testing, tissue culture and radioimmunoassay were used. Results: ① The levels of acetylcholine-dependent vasodilation in hyperglycemia group were significantly decreased (P <001); ② The levels of NO-2, aortic nitric oxide synthase (NOS) (CGMP) content was significantly lower than the control group (P <0 05, P <0 01). CONCLUSIONS: Long-term hyperglycemia can cause severe disorders in the vascular endothelium-derived vasodilator / nitric oxide (EDRF / NO) system, thereby reducing vasodilation