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目的 探讨角质细胞生长因子 (KGF)的辐射防护作用与机制 ,为应用KGF防护肠道辐射损伤提供实验依据。方法 应用正常及不同剂量γ射线损伤的肠上皮细胞 (IEC 6) ,通过在培养体系中加入不同剂量的KGF后检测细胞增殖活性的变化以及凋亡细胞百分率 ,作为KGF辐射防护效应和细胞辐射敏感性变化的指标。结果 正常培养的IEC 6细胞 ,KGF呈剂量依赖型促细胞增殖作用 ;10Gy照射后KGF促细胞增殖作用显著减弱。KGF预处理能显著降低IEC 6细胞辐射敏感性 ,其IP50 ( 50 %增殖抑制照射量 )从对照( 15.3± 0 .6)Gy降至 ( 12 .2± 0 .4 )Gy。结论 KGF具有促肠上皮细胞增殖作用 ,其辐射防护作用表现为降低细胞辐射敏感性 ,抑制辐射诱导的细胞凋亡可能是其作用机制之一。
Objective To investigate the radioprotective effect and mechanism of keratinocyte growth factor (KGF) in order to provide experimental evidence for the protective effect of KGF on radiation damage of intestinal tract. Methods The intestinal epithelial cells (IEC 6) injured by normal and different doses of γ-rays were used to detect the changes of cell proliferative activity and the percentage of apoptotic cells by adding different doses of KGF into the culture system as KGF radiation protective effect and cell radiation sensitivity Indicators of sexual change. Results In IEC 6 cells cultured in a dose-dependent manner, KGF dose-dependently induced the proliferation of IEC 6 cells. The effect of KGF on the proliferation of IEC 6 cells was significantly attenuated after 10 Gy irradiation. KGF pretreatment significantly reduced the radiation sensitivity of IEC 6 cells, and its IP50 (50% inhibition of proliferation) decreased from (15.3 ± 0.6) Gy to (12.2 ± 0.4) Gy. Conclusions KGF can promote the proliferation of intestinal epithelial cells. The radioprotective effect of KGF is to decrease the radiosensitivity of cells and inhibit the apoptosis induced by radiation, which may be one of its mechanisms.