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目的:建立肝癌及癌旁组织miRNA差异表达谱,分析可能与肝癌相关的miRNA。方法:收集肝癌及癌旁组织标本,提取组织RNA,利用miRNA芯片技术分析肝癌组织及其癌旁组织中具有表达差异的miRNA。结果:芯片结果表明具有差异表达的miRNA共2549个,其中部分miRNA具有明显差异。结论:肝癌组织差异表达的miRNA对肝癌发生机制的研究具有重要的参考价值。
Objective: To establish differential expression profiles of miRNA in hepatocellular carcinoma and its adjacent tissues, and to analyze miRNAs that may be associated with hepatocellular carcinoma. Methods: Tissues of hepatocellular carcinoma and its adjacent tissues were collected and tissue RNA was extracted. MiRNAs with differential expression in hepatocellular carcinoma tissues and adjacent tissues were analyzed by miRNA microarray. Results: The results showed that there were 2549 differentially expressed miRNAs, some of which had significant differences. Conclusion: The miRNAs differentially expressed in hepatocellular carcinoma have important reference value for the study on the mechanism of hepatocellular carcinoma.