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为便于临床检验确诊和教学示教,试用活体染色剂进行人毛滴虫的染色及其活动情况观察。 人毛滴虫系于1982年5月底,从上海市第八人民医院门诊的腹泻病人粪便中分离获得,用洛克氏液-鸡蛋-血清(IES)培养基在36±1℃温箱中培养传代至今.选取甲苯胺蓝和中性红两种活体染色剂,分别以洛克氏液稀释配成不同浓度的染色液.取培养48小时的人毛滴虫培养物滴于若干载玻片上,每
In order to facilitate the diagnosis and teaching of clinical test teaching, try the live dye staining of human Trichomonas and observation of their activities. Human trichomoniasis was isolated from the excrement of diarrhea patients in the outpatient clinic of Shanghai No. 8 People’s Hospital at the end of May 1982 and was subcultured in Rockwell-Egg-Serum (IES) medium in a 36 ± 1 ℃ incubator To date, toluidine blue and neutral red two living stains were selected, respectively, dilute with Rockwell solution dubbed a different concentration of staining solution to take cultured 48 hours of human Trichomonas culture dropped on a number of slides, each