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目的研究β肾上腺素受体在豚鼠输尿管ICC细胞上的表达及意义。方法通过离体肌条实验,观察对照组(正常豚鼠肌条)和实验组(Glivec选择性阻断ICC细胞的肌条)在β肾上腺素激动剂作用下输尿管肌条的收缩幅度和频率的变化;采用免疫荧光双标技术检测豚鼠输尿管β肾上腺素受体亚型(β1、β2和β3)在ICC细胞上的表达情况。结果非选择性β肾上腺素激动剂(异丙肾上腺素)作用后,对照组的收缩幅度[(0.070±0.035)g]较实验组的幅度[(0.100±0.028)g]下降显著(P<0.05),频率[(2.480±1.210)/min]较实验组的频率[(7.200±3.580)/min]下降亦显著(P<0.01),实验组在异丙肾上腺素作用下的舒张效应不如对照组显著;免疫荧光双标显示,输尿管ICC细胞仅表达β3受体亚型;选择性β3肾上腺素激动剂(CGP12177)作用后,对照组的收缩幅度[(0.068±0.023)g]和频率[(3.870±0.474)/min]较实验组的幅度[(0.115±0.031)g]和频率[(6.410±1.895)/min]均下降显著(P<0.05),实验组在CGP12177作用下的舒张效应不如对照组显著。结论去甲肾上腺素能神经可能通过激活ICC细胞上的肾上腺素β3受体来调节输尿管收缩与舒张。
Objective To study the expression and significance of β-adrenergic receptor on guinea pig ureter ICC cells. Methods The changes of contractile amplitude and frequency of ureteral myoblasts under the action of β adrenergic agonist were observed by the in vitro muscle strips test in control group (normal guinea pig muscle strips) and experimental group (Glivec selective block ICM muscle strips) The expression of β-adrenergic receptor (β1, β2 and β3) in ureter of guinea pig in ICC cells was detected by immunofluorescence double labeling. Results Compared with the experimental group, the contractile amplitude [(0.070 ± 0.035) g] in the control group decreased significantly (P <0.05) after treatment with non-selective β-adrenergic agonist (isoproterenol) ), The frequency [(2.480 ± 1.210) / min] was significantly lower than that of the experimental group [(7.200 ± 3.580) / min] (P <0.01). The relaxation effect of the experimental group under isoproterenol was not as good as that of the control group (0.068 ± 0.023) g] and the frequency [(3.870 ± 0.023) g) in the ICC cells of the ureter were significantly higher than those of the control group (P <0.05) ± 0.474) / min] (P <0.05). Compared with the control group, the relaxation effect of CGP12177 in the experimental group was not as good as that in the control group ([0.115 ± 0.031] g] and [6.410 ± 1.895 / Group significantly. Conclusion Noradrenergic nerves may regulate ureter contraction and relaxation by activating adrenergic β3 receptors on ICC cells.