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本文以荧光分光光度法分别测定了脂质体液中鬼臼毒素总含量、未包入脂质体内的游离鬼臼毒素含量,及脂质体的包封率。最大激发波长290nm,发射波长633nm,线性范围28.03~448.40μg/L(r=0.9997),回收率在99%以上。本法灵敏度高,专属性强,结果准确。
In this paper, the total content of podophyllotoxin, the content of free podophyllotoxin and the entrapment efficiency of liposomes were determined by fluorescence spectrophotometry. The maximum excitation wavelength was 290 nm, the emission wavelength was 633 nm, and the linear range was 28.03 ~ 448.40 μg / L (r = 0.9997). The recovery rate was above 99%. The law of high sensitivity, specificity, accurate results.