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[Objective]To determine the optimum extracting process of total flavonoids in rhizoma smilacis glabrae. [Methods]On the basis of single factor test and with the yield of total flavonoids in rhizoma smilacis glabrae as index,we determine the optimum extracting process of total flavonoids in rhizoma smilacis glabrae with the method of orthogonal test. [Results]The optimum extracting process of total flavonoids in rhizoma smilacis glabrae is as follows: the ratio of feed liquid is 1∶ 20,the concentration of ethanol is 60%,the temperature of extraction is 95 ℃,extract 2 times by reflux extraction method,2 hours each time. On this condition,the yield of total flavonoids in rhizoma smilacis glabrae reached 8. 34%. [Conclusions] The extracting process is simple for operation,stable and practicable.
[Objective] To determine the optimum extracting process of total flavonoids in rhizoma smilacis glabrae. [Methods] On the basis of single factor test and with the yield of total flavonoids in rhizoma smilacis glabrae as index, we determine the optimum extracting process of total flavonoids in rhizoma smilacis glabrae with the method of orthogonal test. [Results] The optimum extracting process of total flavonoids in rhizoma smilacis glabrae is as follows: the ratio of feed liquid is 1:20, the concentration of ethanol is 60%, the temperature of Extraction conditions are 95 ° C, extract 2 times by reflux extraction method, 2 hours each time. On this condition, the yield of total flavonoids in rhizoma smilacis glabrae reached 8. 34%. [Conclusions] The extracting process is simple for operation, stable and practicable.