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能够准确识别间日疟原虫感染的新的诊断方法对于临床和研究领域均十分有益。作者推出用市售非放射性试剂检测PCR产物的方法,来确定和预测旅游者的间日疟治疗失败。 该法以生物素化的PCR产物与单股RNA探针杂交,此RNA-DNA杂交体被预包被于96孔反应板内的亲合素捕获,加入酶
New diagnostic methods capable of accurately identifying P. vivax infections are of great benefit to both the clinical and research communities. The authors have introduced a method for detecting PCR products using commercially available non-radioactive reagents to determine and predict the failure of tourists for vivax malaria treatment. The method hybridizes biotinylated PCR products with single-stranded RNA probes that have been precoated with avidin in a 96-well reaction plate and added with enzyme