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背景:氧自由基诱导的功能异常可能使心脏、肝脏、肾脏及脑等组织缺血性疾病的主要致病因素,对损伤有保护作用的药物研究成为当前的热点。目的:研究红花黄素(Saffloweryellowpigment,SYP)对氧自由基引起的豚鼠单个心室肌细胞损伤的保护作用。设计:非随机对照的实验研究。地点、材料和干预:本实验在哈尔滨医科大学药学院药理教研室完成。实验选用豚鼠30只,性别不限。以处置前单个心室肌细胞为正常对照,预先应用红花黄素(3.3μg/L)后,给予单个豚鼠心室肌细胞外源性氧自由基(1mmol/L的H2O2)。主要观察指标:采用全细胞膜片钳技术,观察单个豚鼠心室肌细胞动作电位时程(actionpotentialdurationAPD)和L-型钙电流、内向整流钾电流。结果:1mmol/L的H2O2导致豚鼠心室肌细胞损伤,表现为动作电位时程缩短,APD50和APD90分别由正常对照组的(331.2±31.9)ms和(380.8±28.2)ms缩短至(169.5±76.0)ms和(238.4±21.3)ms(n=8,t=3.834,P<0.01),红花黄素(3.3μg/L)能明显改善H2O2诱发的动作电位时程缩短;同时氧自由基H2O2抑制L-型钙电流,+10mV时,L-型钙电流峰值由对照的(-1023.45±74.34)pA减少到(-275.21±38.67)pA(n=6,P<0.001);H2O2(1mmol/L)也明显抑制内向整流钾电流(IK1),指令电位为-120mV时,IK1由对照组的(-2133.5±570
BACKGROUND: The abnormal function induced by oxygen free radicals may make the main pathogenic factors of ischemic diseases in the tissues of the heart, liver, kidneys, and brain, and the study of drugs that have protective effects on the lesions has become a hot spot. Objective: To investigate the protective effect of safflowery-ellow pigment (SYP) on oxygen free radical-induced guinea pig single ventricular myocyte injury. Design: Non-randomized controlled experimental study. Location, Materials, and Interventions: This experiment was performed at the Department of Pharmacology, School of Pharmaceutical Sciences, Harbin Medical University. 30 guinea pigs were selected for the experiment, regardless of gender. Single ventricular myocytes were treated as normal controls, and safflower (3.3 μg/L) was pretreated before exogenous oxygen free radicals (1 mmol/L H2O2) were administered to single guinea pig ventricular myocytes. MAIN OUTCOME MEASURES: Whole cell patch clamp technique was used to observe the actionpotential duration (APD), L-type calcium current, and inward rectifier potassium current in single guinea pig ventricular myocytes. RESULTS: 1mmol/L H2O2 caused damage to ventricular myocytes of guinea pigs, which showed a shortening of action potential duration. APD50 and APD90 were shortened from (331.2±31.9) ms and (380.8±28.2) ms to (169.5±76.0) in the normal control group, respectively. ) ms and (238.4±21.3) ms (n=8, t=3.834, P<0.01). Safflor yellow (3.3 μg/L) significantly improved H2O2-induced action potential duration shortening; meanwhile, oxygen radical H2O2 When L-type calcium current was inhibited, the peak value of L-type calcium current was decreased from control (-1023.45±74.34) pA to (-275.21±38.67) pA (n=6, P<0.001) at +10 mV; H2O2 (1 mmol/L). L) also significantly inhibited inward rectifier potassium current (IK1), instruction potential is -120mV, IK1 from the control group (-2133.5 ± 570