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目的和方法 :利用激光扫描共聚焦显微镜和双波长荧光分光光度计 ,分别观察牛磺酸对无血清培养的单个海马神经细胞和分散的新生大鼠脑神经细胞内Ca2 +浓度 ([Ca2 +]i)的影响 ,并探讨牛磺酸调节神经细胞内钙稳态的作用机理。结果 :牛磺酸主要通过刺激细胞内钙库释放 ,在一定剂量范围内 (0 .0 2～ 6 .4mmol/L)使神经细胞[Ca2 +]i 轻微升高 ,在 0 .4mmol/L时的升钙作用最大 (升钙百分率为 12 .2 0 %± 1.2 4% )。在测定介质中加入钙离子载体A2 3187(10 μmol/L) ,使神经细胞内的钙离子浓度升高 ,若此时加入牛磺酸 (1.6mmol/L) ,则神经细胞内钙离子的浓度下降。结论 :牛磺酸对细胞内钙离子有双向调节作用 ,牛磺酸可能是通过对钙稳态的调节作用来阻止细胞内钙超载 ,保护神经细胞、并发挥其增强动物学习记忆等方面功能的。
PURPOSE AND METHODS: The effects of taurine on the intracellular Ca2 + concentration ([Ca2 +]) in a single hippocampal neuron cultured in a serum-free medium and in scattered neonatal rat brain were observed by laser scanning confocal microscope and dual-wavelength fluorescence spectrophotometer. i), and to explore the mechanism of taurine regulating calcium homeostasis in nerve cells. RESULTS: Taurine increased the [Ca2 +] i of neurons slightly at a dose range (0. 02-6. 4 mmol / L) by stimulating the release of intracellular calcium, and at 0. 4 mmol / L The greatest effect of calcium (calcium percentage was 12.2% ± 1.2 4%). Calcium ionophore A2 3187 (10 μmol / L) was added into the assay medium to increase the concentration of calcium in nerve cells. If taurine (1.6 mmol / L) was added at this time, the intracellular calcium concentration decline. CONCLUSION: Taurine can modulate intracellular Ca2 + in a bidirectional manner. Taurine may prevent intracellular Ca2 + overload and protect nerve cells by modulating Ca2 + homeostasis and play its role in enhancing animal learning and memory .