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目的:通过ITS序列相似性分析、系统发育关系分析和形态分类方法对无菌检验样品检出的两株真菌F162(GU724349)和F927(GU724350)进行了种类鉴定,并与实验室的环境真菌407(GU724347)、413(GU724348)进行同源性分析。方法:对样品的DNA进行扩增和测序,获得真菌ITS基因序列,并与GenBank中相应的序列进行比对分析。结果:真菌F162(GU724349)和F927(GU724350)的ITS基因序列与GenBank中Penicillium sp.的序列相似性均为100%,与实验室的环境真菌407(GU724347)、413(GU724348)的ITS基因序列相似性均分别为93.80%,93.80%,93.63%,93.63%。以NJ法构建的系统发育树显示无菌抽验样品检出的两株真菌与本实验室环境真菌同源性低,两者位于不同亚组。结论:根据ITS序列相似性分析、系统发育关系分析和形态特征观察的结果,将无菌检出菌鉴定为Penicillium sp.。
OBJECTIVE: To identify the two fungi F162 (GU724349) and F927 (GU724350) which were detected in the aseptic test samples by similarity analysis of ITS sequences, phylogenetic relationship and morphological classification, and to identify with laboratory fungi 407 (GU724347), 413 (GU724348) for homology analysis. Methods: The DNA of the sample was amplified and sequenced, and the ITS sequence of the fungus was obtained and compared with the corresponding sequence in GenBank. RESULTS: The ITS sequences of fungi F162 (GU724349) and F927 (GU724350) were 100% identical to those of Penicillium sp. In GenBank. The ITS sequences of fungi 407 (GU724347) and 413 (GU724348) Similarities were 93.80%, 93.80%, 93.63%, 93.63% respectively. The phylogenetic tree constructed by NJ method showed that the two strains of fungi which were detected by aseptic sampling had low homology with the fungi in this laboratory environment, both of which were located in different subgroups. Conclusion: Based on the similarity analysis of ITS sequences, phylogenetic analysis and morphological observation, the bacterium was identified as Penicillium sp.