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开展穗相关突变基因的定位与克隆有利于解析穗发育的分子调控机理,对水稻超高产分子设计育种具有重要理论价值。为探究水稻穗发育分子机理,在甲基磺酸乙酯(EMS)诱变的水稻突变体库中鉴定到了一个能稳定遗传的秃穗突变体nsp1。遗传分析表明,该突变性状是由一对单隐性核基因控制。通过SSR和STS分子标记对F2分离群体进行遗传定位,最终将NSP1精细定位在4号染色体分子标记zd38和zd30之间约41.6 Kb的区间内,发现该区间共有7个预测基因,其中LOC_Os04g56780为一个与分生组织发育相关的WUSCHEL的同源基因,可能为NSP1的候选基因。本研究结果为进一步克隆该基因和功能分析奠定了基础。
The mapping and cloning of spike-related mutated genes is helpful to analyze the molecular regulation mechanism of spike development and has important theoretical value for the design and breeding of super-high molecular weight rice. In order to explore the molecular mechanism of rice panicle development, a stably inherited bald spike mutant nsp1 was identified in the rice mutant library mutated by ethyl methanesulfonate (EMS). Genetic analysis showed that the mutant trait was controlled by a pair of single recessive nuclear genes. Through SSR and STS molecular markers to F2 segregating population, the NSP1 locus was finely mapped on the interval of 41.6 Kb between chromosome markers zd38 and zd30 on chromosome 4, and there were 7 predicted genes in this interval, of which LOC_Os04g56780 was one The homologous gene of WUSCHEL associated with the development of meristem may be a candidate gene of NSP1. The results of this study laid the foundation for further cloning of this gene and functional analysis.