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背景与目的:鼻腔鼻窦非霍奇金淋巴瘤(non-Hodgkin’s lymphoma,NHL)的患病率和免疫表型组成具有地域性差异。本研究探讨中国广州地区57例鼻腔鼻窦NHL免疫表型及其与EB病毒(Epstein-Barrvirus,EBV)感染的关系。方法:收集2000年4月1日至2006年10月31日中山大学肿瘤防治中心病理科57例鼻腔鼻窦NHL标本。免疫组化染色确定免疫表型,EBER原位杂交及PCR检测EBV感染情况。结果:在同期诊断的1412例NHL中,71例(5.03%)发生于鼻腔鼻窦,其中仅有57例适用于本研究。57例鼻腔鼻窦NHL患者中,男性38例,女性19例,年龄3～75岁,中位年龄50岁;44例(77.19%)为鼻型NK/T细胞淋巴瘤,其中37例(84.09%)为EBV+/CD56+NK细胞肿瘤,7例(15.91%)为EBV+/CD56-细胞毒性T细胞表型;11例(19.30%)为B细胞淋巴瘤,其中6例为弥漫大B表型,2例为Burkitt(Burkitt样)淋巴瘤(EBV+),1例为髓外浆细胞瘤(EBV+),1例为MALT淋巴瘤(EBV-),1例为小淋巴细胞性淋巴瘤(EBV-);2例(3.51%)为外周T细胞淋巴瘤(EBV-)。37例适用DNA检测的病例中,25例(67.57%)感染缺失型LMP1(del-LMP1)EBV株,12例(32.43%)感染野生型LMP1(wt-LMP1)EBV株。结论:鼻腔鼻窦NHL最常见的类型为鼻型NK/T细胞淋巴瘤,可进一步分为EBV+/CD56+NK细胞及EBV+/CD56-细胞毒性T细胞表型。NK/T细胞淋巴瘤均感染了EBV,EBV株主要为del-LMP1型。
BACKGROUND & OBJECTIVE: The prevalence and immunophenotypic composition of nasal sinus non-Hodgkin’s lymphoma (NHL) have regional differences. This study was to investigate the immunophenotyping of NHL in 57 cases of nasal sinus and its relationship with Epstein-Barr virus (EBV) infection in Guangzhou, China. Methods: 57 cases of nasal sinus NHL specimens collected from April 1, 2000 to October 31, 2006, Department of Pathology, Sun Yat-sen University Cancer Center were collected. Immunohistochemistry was used to determine the immunophenotype, EBER in situ hybridization and PCR to detect EBV infection. RESULTS: Of the 1412 NHLs diagnosed during the same period, 71 (5.03%) occurred in nasal sinuses, of which only 57 were eligible for this study. Fifty-seven cases of nasal sinus NHL patients included 38 males and 19 females, aged 3 to 75 years old, with a median age of 50 years. Forty-four patients (77.19%) had nasal NK / T cell lymphomas, of which 37 (84.09% ) Were EBV + / CD56 + NK cell tumors, 7 (15.91%) were EBV + / CD56- cytotoxic T cell phenotypes; 11 (19.30%) were B cell lymphomas, of which 6 were diffuse large B phenotypes, 2 were Burkitt-like lymphoma (EBV +), 1 was extramedullary plasmacytoma (EBV +), 1 was MALT lymphoma (EBV-) and 1 was small lymphocytic lymphoma (EBV-) ; 2 cases (3.51%) were peripheral T cell lymphoma (EBV-). Of the 37 DNA tests, 25 (67.57%) infected LMP1 (del-LMP1) EBV strains and 12 (32.43%) infected wild type LMP1 (wt-LMP1) EBV strains. Conclusion: The most common type of nasal sinus NHL is nasal NK / T cell lymphoma, which can be further divided into EBV + / CD56 + NK cells and EBV + / CD56- cytotoxic T cell phenotype. NK / T cell lymphoma are infected with EBV, EBV strains mainly del-LMP1 type.