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目的根据对比结果,找出一种理想的实验方法进行梅毒筛查,防止漏查、误检,提高检测效率和准确度。方法采用酶联免疫吸附试验(ELISA)与甲苯胺红卡片试验(TRUST)对比对14 608名出入境人员及82名梅毒病人进行检测,对 ELISA 和 TRUST 检测出的阳性样品均用螺旋体致敏明胶颗粒凝集试验(TPPA)进行验证。结果对14 608名出入境人员进行梅毒筛查,ELISA 试验与 TPPA 试验完全符合,TRUST 试验有11例不符合;对82名梅毒病人检测,TRUST 试验有1例不符合,ELISA 试验完全符。结论 ELISA 法筛查梅毒具有敏感性高、特异性好、自动化程度高、易于大批量检测等优点,适合于出入境人员进行梅毒检测。
Objective According to the comparison results, find out an ideal experimental method for syphilis screening to prevent missed inspection, false positives and improve the detection efficiency and accuracy. Methods 14 608 exit-entry and 82 syphilis patients were tested by enzyme-linked immunosorbent assay (ELISA) and toluidine red card test (TRUST). The positive samples detected by ELISA and TRUST were detected with spirochetes Particle agglutination test (TPPA) to verify. Results A total of 14 608 entry and exit officers were screened for syphilis. The ELISA and TPPA tests were in good agreement with TRUST. Eleven patients did not fit the TRUST test. One patient did not meet the TRUST test in 82 syphilis patients, and the ELISA test was completely consistent. Conclusion ELISA screening of syphilis has the advantages of high sensitivity, good specificity, high degree of automation and easy detection in large quantities. It is suitable for the detection of syphilis among immigration officers.