论文部分内容阅读
目的探讨IL-6基因-572C/G多态性与小儿全身炎症反应综合征(SIRS)的关系。方法选用病例-对照的研究方法,以本院53例SIRS患儿为SIRS组,并随机挑选50例健康儿童为健康对照组(所有儿童均为汉族,来自广东省深圳市),采用酚/氯仿法提取2组儿童外周血白细胞DNA,并制备血清。自行设计引物,应用聚合酶链反应对2组研究对象的IL-6基因启动子-572位点的特异性片段进行扩增,再应用限制性片段长度多态性法对扩增的特异性片段用限制性内切酶进行酶切。酶切产物采用琼脂糖凝胶电泳法进行检测,分析2组儿童IL-6基因-572位点基因型及等位基因频率的分布。应用酶联免疫吸附法(ELISA)检测2组儿童血清IL-6水平,观察不同基因型对其血清IL-6水平的影响。采用SPSS 13.0软件进行数据分析。结果 IL-6基因-572位点基因型和等位基因频率在2组间分布差异均有统计学意义(Pa<0.05);SIRS组GG基因型和G等位基因频率均显著高于健康对照组(Pa<0.05);携带G等位基因个体患SIRS的风险约是C等位基因型个体的5.91倍(95%CI为2.76~12.64);SIRS组血清IL-6水平显著高于健康对照组(P<0.05);G等位基因携带者血清IL-6水平显著高于CC基因携带者(P<0.05)。结论 IL-6基因-572C/G多态性与中国汉族儿童SIRS发病密切相关;血清IL-6水平可能受其基因多态性的影响。
Objective To investigate the relationship between -572C / G polymorphism of IL-6 gene and systemic inflammatory response syndrome (SIRS) in children. Methods A case-control study was conducted in which 53 children with SIRS in our hospital were selected as SIRS group and 50 healthy children were randomly selected as healthy control group (all Han children from Shenzhen, Guangdong Province) with phenol / chloroform France two groups of children’s peripheral blood leukocyte DNA, and preparation of serum. Primers were designed by ourselves. Polymerase chain reaction (PCR) was used to amplify the specific fragment of IL-6 gene promoter-572 site in two groups of subjects. The specific fragment amplified by restriction fragment length polymorphism Restriction enzyme digestion. The digested products were detected by agarose gel electrophoresis, and the distribution of genotype and allele frequency at IL-6 gene -572 locus was analyzed. Serum IL-6 levels were measured by enzyme-linked immunosorbent assay (ELISA) in two groups of children, and the effects of different genotypes on serum IL-6 levels were observed. SPSS 13.0 software for data analysis. Results The frequency of genotype and allele at -572 locus in IL-6 gene was significantly different between the two groups (P <0.05). The frequencies of GG genotype and G allele in SIRS group were significantly higher than those in healthy controls Group (P <0.05). The risk of SIRS in individuals with G allele was 5.91 times (95% CI, 2.76-12.64) compared with individuals with C allele. The serum IL-6 level in SIRS group was significantly higher than that in healthy controls (P <0.05). The level of serum IL-6 in G allele was significantly higher than that in CC gene (P <0.05). Conclusions The -572C / G polymorphism of IL-6 gene is closely related to the onset of SIRS in Chinese Han children. Serum IL-6 level may be affected by its genetic polymorphism.