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为提高海洋红球菌SY095发酵产生物表面活性剂的能力,研究发酵培养基中碳源及氮源的影响,并通过响应面分析法优化培养基各组分的含量。首先通过单因素实验,确定培养基最佳碳源、氮源分别为大豆油和尿素,在此基础上,利用Plackett-Burman实验设计筛选出2个对表面活性剂产量有显著影响的因子为尿素和K2HPO_4,通过最陡爬坡实验和响应面分析法对显著影响因子进行优化,获得海洋红球菌产表面活性剂的最佳培养基配方为:大豆油2%(体积分数),尿素2.35 g·L~(-1),K_2HPO_40.69 g·L~(-1),KH_2PO_41.0 g·L~(-1),Mg SO_40.5 g·L~(-1)。使用该优化培养基配方,海洋红球菌发酵液的表面张力为29.254 m N·m~(-1),与预测值接近,表面张力活性比优化前提高了12.9%。
In order to improve the ability of Rhodococcus SY095 to produce surfactant, the effects of carbon source and nitrogen source in fermentation medium were studied, and the content of each component in culture medium was optimized by response surface analysis. First of all, through single factor experiments, the best carbon source in the medium was determined, and the nitrogen sources were soybean oil and urea respectively. Based on this, two factors influencing the yield of surfactant were selected by Plackett-Burman design. And K2HPO_4, and the most influential factors were optimized by steepest ascent test and response surface analysis. The optimal medium for obtaining Rhodococcus sp. Production medium was 2% (volume fraction) of soybean oil and 2.35 g of urea L -1, K 2 HPO 40.69 g · L -1, KH 2 PO 41.0 g · L -1 and MgSO 40.5 g · L -1. The surface tension of marine Rhizoctonia fermentation broth was 29.254 mN · m -1 using this optimized medium formula, which was close to the predicted value and the surface tension activity was increased by 12.9% compared with that before optimization.