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[目的]旨在寻求一种微量、快速提取小麦DNA的方法。[方法]以小麦幼叶为材料,分别用改良CTAB法、改良SDS法和沸水浴法提取小麦叶片总DNA,通过琼脂糖凝胶电泳法、紫外吸收法和PCR检测DNA完整性和纯度。[结果]改良CTAB法提取的小麦基因组DNA质量和纯度高、无降解,每人每天可以轻松提取200个DNA样品,对转基因植株的PCR检测显示扩增目标条带清晰一致,无假阳性,试验结果理想。改良SDS法所提DNA纯度和浓度虽略不如改良CTAB法,但仍然符合实验要求。沸水浴法提取量少且杂质多,PCR无有效扩增,结果不理想。[结论]改良CTAB法提供了一种简便、快速微量小麦DNA提取方法,适用于PCR和其他分子生物学研究。
[Objective] The aim was to seek a method to extract wheat DNA rapidly and trace amount. [Method] With wheat young leaves as material, the total DNA of wheat leaves were extracted by improved CTAB method, modified SDS method and boiling water bath method. DNA integrity and purity were detected by agarose gel electrophoresis, UV absorption and PCR. [Result] The quality and purity of wheat genomic DNA extracted by modified CTAB method was high without degradation. 200 DNA samples could be easily extracted per person per day. PCR detection of transgenic plants showed that the amplification target bands were clear and consistent without false positive test The result is ideal. Although the purity and concentration of DNA proposed by the modified SDS method are slightly less than that of the modified CTAB method, they still meet the experimental requirements. Less boiling water bath extraction method and more impurities, PCR no effective amplification, the result is not satisfactory. [Conclusion] The improved CTAB method provided a simple and rapid DNA extraction method for trace wheat, suitable for PCR and other molecular biology studies.