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对8个光敏感雄性不育籼稻杂种一代及其亲本花药培养的研究发现,光敏不育粳稻虽然为雄性不育,但其愈伤组织诱导率与植株再生能力不在一般正常粳稻品种之下,为籼稻“新会粘”的28倍。绝大多数光敏不育之籼粳杂种的花药培养能力也是十分高的。在起源于体细胞及花药的光敏不育水稻之再生植株共1146株试管苗中,发现少数在5月下旬及6月上旬抽穗的已表现为完全雄性不育。这些材料之幼穗的第二枝梗分化时(4月下旬至5月上旬)之日长尚不足13小时,远在农垦58S的临界日长14小时之下。在8个组合的籼粳光敏杂种一代花粉植株中,有的组合完全表现为粳型(组合4及7,表2),有的完全表现为籼型(组合2及6),多数则两者兼有。在全部80个愈伤无性系中,单倍体20个,二倍体58个,多倍体2个。58个二倍体无性系申不育的为20个,占34.5%。其中在晚造短日照条件下能转变为可育的有11个,占二倍体的19%,占二倍体不育的55%。所有光敏不育花粉植株,在早造条件下I-KI之花粉染色率与结实率均为0%,在晚造(9月份之后)条件下,能转换为可育。但不同组合、不同无性系之间育性转换的频率与稳定性差异颇大。
The study on the anther culture of 8 photosensitive male-sterile indica rice hybrids and their parents showed that although the photo-sterile male sterile rice was male-sterile, its callus induction rate and plant regeneration ability were not under the normal normal japonica rice varieties, and were Indica “new will stick” 28 times. The vast majority of photosensitive male sterile indica japonica hybrid anther culture ability is very high. In a total of 1146 plantlets of regenerated plants derived from somatic cells and anthers of photo-sensitive sterile rice, it was found that a few of them had been completely male sterile in late May and early June. The second branch of young panicles of these materials had a duration of less than 13 hours on the day of differentiation (late April to early May), well below the critical day of Nongken 58S for 14 hours. Among the eight combinations of pollen plants of Indica-Japonica Photosensitive Hybrids, some combinations showed complete japonica (combinations 4 and 7, Table 2), others completely indicted (combinations 2 and 6), most of them Both Of all 80 callus, 20 were haploid, 58 were diploid and 2 were polyploid. 58 diploid clones were infertile 20, accounting for 34.5%. Among them, 11 were fertile in late-day short-day conditions, accounting for 19% of diploid and 55% of diploid infertility. All photosensitive sterile pollen plants, pollination rate and seed setting rate of I-KI were 0% under the conditions of early generation, and could be changed to fertile at late stage (after September). However, the frequency and stability of fertility transformation between different combinations and different clones vary greatly.