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1 材料和方法 1.1 参试品种。GK—12、中R108、中棉所17号,以中棉所17号作对照(cK)。 1.2 供试棉铃虫。由江苏农科院提供的1龄标准幼虫,即从卵孵化后用人工饲料上饲养2d(天)的幼虫。 1.3 接种。在现蕾期(6月20日左右)采摘顶部展开嫩叶,花铃期(7月20日和8月20日)采摘上部果枝顶尖展开嫩叶,保留嫩叶叶柄2cm左右,采摘后将各单株叶片分别装在各自的塑料袋内,标明品种名称及株号,以便分别观察各单株的抗虫
1 materials and methods 1.1 test varieties. GK-12, R108, China Cotton Institute No. 17, with CIMC No. 17 as a control (cK). 1.2 for test bollworm. The first instar larvae, provided by Jiangsu Academy of Agricultural Sciences, are larvae that are kept for 2 days (days) from hatching with artificial diet. 1.3 vaccination. In the budding period (around June 20) picking the top to develop young leaves, flower bell period (July 20 and August 20) picking the top branches of the top to start young leaves, leaving the leaves of petioles about 2cm, after picking each The leaves of individual plants were respectively packed in their respective plastic bags, indicating the name of the variety and the plant number so as to observe the resistance of each individual plant