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通过建立头孢曲松钠所致人肠道菌群紊乱体外模型，结合菌群的选择性培养、平板计数及短链脂肪酸分析，研究经典名方生脉散对肠道菌群失调的调节作用。研究结果显示，生脉散高中低剂量（16．5，11．2，5．5 mg／mL）组能显著抑制肠杆菌、肠球菌的增殖，促进肠道益生菌乳酸杆菌、梭菌增殖。与模型组相比，给予中剂量生脉散培养24 h 后，肠球菌、肠杆菌增殖分别被抑制了24．9％和19．1％，乳酸杆菌和梭菌的增殖分别提高了22．3％和25．8％。同时生脉散各剂量组均能显著促进肠道菌群产生丙酸和乙酸，抑制潜在致病菌的增殖，促进失衡肠道菌群向正常菌群结构发展。“,”To study the regulation effect of Sheng-mai San (SMS)on intestinal dysbacteriosis,ceftriaxone sodium was used to induce intestinal dysbacteriosis in vitro,the bacteria were cultured in the selective medium and enu-merated by plat counting method,and the short chain fatty acid were also analyzed .The results showed that SMS (16.5,11.2,5.5 mg/mL)could significantly inhibit the proliferation of potentially harmful bacteria such as Enterococci,Enterobacteriaceae and promote the proliferation of Lactobacillus and Clostridum.Compared with the model group,the proliferation of Enterococci and Enterobacteriaceae was inhibited by 24.9% and 19.1%,while the proliferation of Lactobacillus and Clostridum was increased by 22.3% and 25.8% respectively in the middle-concentration of SMS group (11.2 mg/mL)at 24 h.Meanwhile,SMS in different concentration significantly increase the accumulation of acetic acid and propionic acid generated by gut microbes which could significantly inhibit the proliferation of harmful bacteria.And SMS showed prebiotic effects on intestinal microflora imbalance induced by antibiotics in vitro.