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以药物Verapamil（VER）与人血清白蛋白（HSA）相互作用体系中游离的药物对映体浓度定量测定为目标，建立了一项适用于相互作用研究的液相预柱毛细管电泳（LPC－CE）技术。通过对该技术的考察，确定了这项技术的定量可靠性。在生理pH值条件下（pH7.4，离子强度I=0．17），使药物与人血清白蛋白达到结合平衡。在毛细管电泳手性拆分[pH2.5缓冲浪；三甲基-β-环糊精（TM－β-CD）浓度为45mmol／L]柱（32cm×50μm）内预先注入一段生理pH缓冲液，形成一段液相预柱（2．8cm）。在顶柱中，利用蛋白和药物在生理pH下的电泳特性差异，使HSA不进入手性拆分区域，而药物以平衡浓度进入拆分系统。结合毛细管电泳前沿分析技术，药物对映体在相互作用体系中的游离浓度的测定通过手性拆分实现。对7个不同浓度比的药物一白蛋白样品体系进行考察，具有良好的重现性（相对标准偏差RSD=2．17％～5．02％，n=4）;定量分析的相对误差在1．4％～5．8％范围内。
Aim To determine the concentration of free drug enantiomers in the interaction system of drug Verapamil (VER) and human serum albumin (HSA), a liquid phase pre-column capillary electrophoresis (LPC-CE )technology. Through the investigation of this technology, the quantitative reliability of this technology has been confirmed. Under physiological pH conditions (pH7.4, ionic strength I = 0.17), the drug and human serum albumin reached a binding equilibrium. A section of physiological pH buffer (30 cm × 50 μm) was pre-injected into the capillary electrophoresis chiral resolution [pH2.5 buffer; TM-β-CD concentration 45 mmol / , Forming a liquid phase precolumn (2.8 cm). In the top column, taking advantage of differences in electrophoretic properties of proteins and drugs at physiological pH, HSA does not enter the chiral resolution region and the drug enters the resolution system at equilibrium concentrations. In combination with capillary electrophoresis, the determination of the free concentration of the drug enantiomer in the interaction system was achieved by chiral resolution. Seven different concentrations of drug-albumin sample system were investigated, with good reproducibility (relative standard deviation RSD = 2.17% ~ 5.02%, n = 4); the relative error of quantitative analysis of 1 .4% ~ 5.8% range.