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用60～100keV氮离子和75keV碳离子的不同剂量注入甜菊干种子,并与γ射线进行比较,研究其M_1代生物学效应和M_2代突变。结果表明,离子束能诱发甜菊染色体结构变异,抑制有丝分裂活动。随着注入离子的能量和剂量提高,染色体畸变细胞率呈增加趋势。有丝分裂的剂量效应不明显,其能量效应表现在75keV与60和100kev的差异。离子束对甜菊的M_1损伤效应低于γ射线的,而诱发的M_2突变高于γ射线的。碳离子诱发的染色体畸变细胞率和M_2有益突变频率均高于氮离子的。离子注入杂交一代丰_1×日原和日原×丰_2的诱变效应大于济宁和丰_2。
The seeds of Stevia rebaudiana were infused with different dosages of 60 ~ 100keV nitrogen ions and 75keV carbon ions, and compared with γ ray, the biological effects of M_1 generation and M_2 generation mutation were studied. The results showed that ion beam could induce the structural variation of stevia chromosome and inhibit the mitotic activity. As the energy and dose of ion implantation increased, the rate of chromosomal aberrations increased. The dose effect of mitosis is not obvious, and its energy effect is shown in the difference between 75keV and 60 and 100kev. The effect of ion beam on M_1 of stevia was lower than that of γ-rays, while the M_2 mutation induced by ion beams was higher than that of γ-rays. Carbon ion-induced chromosomal aberration cell rate and M 2 beneficial mutation frequency were higher than nitrogen ion. The mutagenic effects of ion implantation hybrid generation Feng_1 × Nipponbare and Nipponbare × Feng_2 were greater than those of Jining and Feng_2.