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In a previous paper, 4 hybridoma cell lines (1B3, 2D5, 2A4 and 2B6) secreting antibodiesagainst heavy chain of hmnan IgG were reported. In this paper a more detailed study of theseMcAbs was introduced. First of all they were studied with double immunodiffusion test inagarose and it was demonstrated that they were reactive against 3 distinct antigenic deter-minants of human IgG. This result was also confirmed by ELISA competition test. TheseMeAbs were further analysed for human IgG subclass proteins and it was found that 1B3McAb gave positive reaction with all subclasses of IgG while the other 3 possessed confinedsubclass reactivity, i.e. McAb 2A4 did not react with IgG3 (ealled non-IgG3 McAb), andboth McAbs 2D5 and 2B6 did not react with IgG4 (called non-IgG4 McAb). By agarose immunodiffusion test it was shown that: (i) Only transparent precipitation linewas formed when McAb reacted with IgG antigen, however, in agarose containing 2% PEG-6000, the transparent precipitation line becomes opaque; (ii) When
In this paper a more detailed study of theseMcAbs was introduced. First of all they were studied with double immunodiffusion test (1B3, 2D5, 2A4 and 2B6) secreting antibodiesagainst heavy chain of hmnan IgG were reported. This result was also confirmed by ELISA competition test. TheseMeAbs were further analysed for human IgG subclass proteins and it was found that 1B3McAb gave positive reaction with all subclasses of IgG while the other 3 possessed confined subsubclass reactivity, ie McAb 2A4 did not react with IgG3 (ealled non-IgG3 McAb), andboth McAbs 2D5 and 2B6 did not react with IgG4 (called non-IgG4 McAb). By agarose immunodiffusion test it was either (i) Only transparent precipitation line was formed when McAb reacted with IgG antigen, however, in agarose containing 2% PEG-6000, the transparent precipitation line became opaque;