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目的观察白藜芦醇(resveratrol,Res)的抗炎作用,分析抗炎效应与氨基脲敏感性胺氧化酶(semicarbazide sensitive amine oxidase,SSAO)的关系,探讨Res抗炎作用新机制。方法将雄性新西兰兔34只,随机分成对照组(n=4)、脂多糖(lipopolysaccharide,LPS)组(n=6)、Res预防组(30、60、120mg/kg,n=6)和2-溴乙胺(2-bromoethylamine,2-BEA)组(n=6)。对照组不做处理,其它各组分别给予1%二甲基亚砜(dimethyl sulfoxide,DMSO,ip)、Res(30、60、120mg/kg,ip)和2-BEA(10mg/kg,iv),并立即进行气管插管灌注LPS建立急性肺损伤的动物模型,。ELISA法测定16h血清IL-1β、IL-6、PGE2、iNOS等炎症因子水平,高效液相色谱法测定16h血浆SSAO活性。结果对雄性新西兰兔气管插管灌注LPS,实验动物血清IL-1β、IL-6、iNOS水平、血浆SSAO活性明显高于对照组(P<0.05);三个剂量的Res降低LPS所致的炎症模型动物血清IL-1β、IL-6、PGE2、iNOS水平、血浆SSAO活性,其中60、120mg/kg Res明显抑制炎症反应(P<0.05)。三个剂量的Res明显降低LPS所致的炎症模型动物肺湿重/干重比值(W/D,P<0.05),不同程度的减轻LPS诱导的急性肺损伤程度。结论 Res具有减轻模型动物急性肺损伤效应,降低动物血清IL-1β、IL-6、PGE2、iNOS等炎症因子水平,抑制动物血浆SSAO活性,其抗炎效应与SSAO活性降低有关。
Objective To observe the anti-inflammatory effect of resveratrol (Res) and the relationship between anti-inflammatory effect and semicarbazide sensitive amine oxidase (SSAO), and to explore a new mechanism of Res anti-inflammatory effect. Methods Thirty - four male New Zealand rabbits were randomly divided into control group (n = 4), lipopolysaccharide group (n = 6), Res group (30,60,120 mg / kg, n = 6) - 2-bromoethylamine (2-BEA) group (n = 6). The control group was given no treatment and the other groups were given dimethyl sulfoxide (DMSO, ip), Res (30,60,120 mg / kg, ip) and 2-BEA , And immediately intratracheal intratracheal instillation of LPS to establish acute lung injury in animal models. Serum levels of IL-1β, IL-6, PGE2, iNOS and other inflammatory cytokines were measured by ELISA. Plasma SSAO activity was measured by high performance liquid chromatography. Results The levels of IL-1β, IL-6, iNOS and plasma SSAO in male New Zealand rabbits were significantly higher than those in control group (P <0.05). Three doses of Res reduced the inflammation induced by LPS The levels of IL-1β, IL-6, PGE2, iNOS and plasma SSAO in model animals were significantly lower than those in control group (P <0.05). Three doses of Res significantly reduced LPS-induced lung wet / dry weight ratio (W / D, P <0.05), and relieved LPS-induced acute lung injury to varying degrees. Conclusions Res can relieve the acute lung injury of model animals and decrease the levels of IL-1β, IL-6, PGE2, iNOS and other inflammatory factors, and inhibit the activity of SSAO. The anti-inflammatory effect is related to the decrease of SSAO activity.