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目的建立应用超顺磁性氧化铁(SPIO)分子探针氨基硅烷Fe3O4纳米颗粒标记人肺腺癌的裸鼠移植瘤模型,行磁共振成像和病理观察,探讨其临床价值。方法制备氨基硅烷Fe3O4纳米颗粒,分别将标记和未标记肺腺癌细胞株植入裸鼠皮下,观察其成瘤情况,行MR扫描和病理观察。结果标记及未标记纳米分子探针SPIO的肿瘤模型均成功建立,其中前者肿瘤较后者在MR成像中信号强度有明显变化,在T1WI、T2WI、FGR/20°和FGR/70°四个序列中,以FGR/20°变化最明显。病理切片普鲁士兰染色可见肿瘤细胞及坏死组织内有铁染色。结论氨基硅烷Fe3O4纳米颗粒标记人肺腺癌细胞建立裸鼠移植瘤模型稳定可靠,应用磁共振可以对其进行活体监测,提示在临床上具有良好的潜在性应用前景。
OBJECTIVE: To establish a nude mouse xenograft model of human lung adenocarcinoma using aminosilane Fe3O4 nanoparticle labeled with SPIO probe, and to investigate its clinical value by magnetic resonance imaging and pathological examination. Methods Aminosilane Fe3O4 nanoparticles were prepared. The labeled and unlabeled lung adenocarcinoma cell lines were implanted subcutaneously in nude mice. The tumorigenesis was observed. MR scanning and pathological examination were performed. RESULTS: The tumor markers of both the labeled and unlabeled nanoparticle probes SPIO were successfully established. Among them, the signal intensity of the former tumor was significantly changed in MR imaging. The intensity of signal intensity in T1WI, T2WI, FGR / 20 ° and FGR / 70 ° In the FGR / 20 ° changes the most obvious. Pathological Prussian blue staining of tumor cells and necrotic tissue with iron staining. Conclusion Aminosilane Fe3O4 nanoparticles labeled human lung adenocarcinoma cells in nude mice model of transplanted tumor is stable and reliable, the use of magnetic resonance imaging can be monitored in vivo, suggesting that the clinical application has a good potential.