论文部分内容阅读
目的 了解恙螨幼虫体内恙虫病东方体自然感染率与恙虫病流行的关系 ,为恙虫病的监测与防治提供理论依据。方法 1974～ 1982年福建省 49个点捕获的野鼠体表采集恙螨幼虫 910 93只 ,同种为一组用乙醇保存 ,以 10～ 5 0只为一份 ,用东方体 5 6 k Da基因所构进的引物进行 PCR扩增。将结果与 90年代捕获的恙螨幼虫 PCR检测结果比较 ,结合相应年代的恙虫病发病情况进行分析。结果 1974～ 1981年捕获地里纤恙螨 2 9份、小板纤恙螨 9份 ,高湖纤恙螨 3份、苍白纤恙螨 12份、于氏纤恙螨 1份、中华无前纤恙螨 1份 ,PCR检测恙螨幼虫体内东方体 DNA均为阴性。1982年 12月采集的小板纤恙螨 1份 ,1991年 6 - 7月采集的地里纤恙螨 1份 ,检测到东方体 DNA。结果表明 1982年前恙螨幼虫体内东方体自然感染率极低。结论 恙螨幼虫体内东方体的自然感染率高低与恙虫病流行有密切关系。
Objective To understand the relationship between the natural infection rate of Orientia tsutsugamushi and Orientia tsutsugamushi in the chigger mite larva and to provide a theoretical basis for the monitoring and prevention of tsutsugamushi disease. Methods A total of 910 93 chigger mite larvae were collected from 49 rodents captured in Fujian Province from 1974 to 1982. One group was kept in ethanol, and the other was 10 to 50. The 5 6 kDa gene The constructed primers were PCR amplified. The results were compared with the PCR results of the chigger mite larvae captured in the 90s and the incidence of scrub typhus in the corresponding years. Results From 1974 to 1981, 19 malachite mites, 9 small chibi mites, 3 high chick mite mites, 12 pale chigger mites, 1 chigger mite, 1 chigger mite, PCR detection of chigger mite larvae Oriental body DNA were negative. In December 1982 collected 1 small chigger mite 1, collected from June to July 1991 in the ground chigger mite 1, detected in the Oriental body DNA. The results showed that the natural infection rate of Oriental body of chigger mite larvae before 1982 was extremely low. Conclusion The natural infection rate of Oriental body in chigger mite larvae is closely related to the epidemic of scrub typhus.