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The effect of Tb3+ ion on the Activity Index (RZ=A403/A275) of HRP was investigated by a combination of ultraviolet-visible, FT-infrared and X-ray photoelectron spectroscopic techniques. It was found that Tb3+ ion could bond to the O and/or N group of the amides in the polypeptide chains of HRP, leading to a decrease in the enzymatic activity index of HRP.
The effect of Tb3 + ion on the Activity Index (RZ = A403 / A275) of HRP was investigated by a combination of ultraviolet-visible, FT-infrared and X-ray photoelectron spectroscopic techniques. It was found that Tb3 + ion could bond to the O and / or N group of the amides in the polypeptide chains of HRP, leading to a decrease in the enzymatic activity index of HRP.