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采用基因工程技术，构建出前列腺组织特异性的基因载体新型Ｃ３（１）。利用该载体和转基因技术，培殖出人ｂｃｌ２基因的前列腺组织特异性转基因鼠。用ＲＮＡ酶保护法和Ｗｅｓｔｅｒｎ杂交检测发现，雄鼠中转基因人ｂｃｌ２的ｍＲＮＡ和蛋白只在前列腺和睾丸中有表达。ＲＮＡ组织原位杂交检测证实人ｂｃｌ２在鼠前列腺上皮细胞中的过量表达。该实验构建和检测了新型Ｃ３（１）作为前列腺组织特异性的基因启动载体的可行性，为前列腺疾病基因治疗研究打下了基础。
Using genetic engineering technology, we constructed a novel C3 (1) gene expression vector of prostate tissue. Using the vector and transgenic technology, human bcl 2 gene-specific prostate tissue-specific transgenic mice were cultured. RNase protection and Western blot detection revealed that the mRNA and protein of bcl-2 gene in transgenic mice were only expressed in prostate and testis. RNA in situ hybridization test confirmed the human bcl 2 overexpression in rat prostatic epithelial cells. This experiment constructed and tested the feasibility of novel C3 (1) as a priming vector for prostate tissue-specific gene and laid the foundation for the gene therapy research of prostatic diseases.