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文中基于高效、生物兼容性纳米结构银膜,采用便携式拉曼光谱仪分别对10个健康人和10个急性粒白血病患者的氧合血红蛋白进行了表面增强拉曼散射(SERS)光谱的研究。实验发现,健康人与急性粒白血病患者的氧合血红蛋白SERS光谱存在显著差异:(1)健康人氧合血红蛋白SERS光谱中位于340cm-1附近很弱的拉曼峰在急性粒白血病患者的SERS光谱中变的很强;(2)健康人氧合血红蛋白SERS光谱中位于655 cm-1附近的拉曼峰在急性粒白血病患者SERS光谱中蓝移到670 cm-1附近,同时在727cm-1附近出现一个新的拉曼峰;(3)对健康人和急性粒白血病患者氧合血红蛋白SERS光谱中位于472、814、1335、1423和1588 cm-1处的拉曼峰相对强度比研究发现,相对强度比I814/I472,I1335/I472、I1423/I472和I1588/I472可以作为较好的区分健康人和急性粒白血病患者的SERS光谱诊断指标,这为基于SERS光谱技术进行急性粒白血病诊断提供了初步实验依据。
In this paper, surface enhanced Raman scattering (SERS) spectra of oxyhemoglobin in 10 healthy individuals and 10 acute leukemia patients were studied by portable Raman spectrometer based on highly efficient and biocompatible nanostructured silver films. It was found that the SERS spectra of oxygenated hemoglobin were significantly different between healthy and acute myeloid leukemia patients. (1) The SERS spectra of SERS spectra of healthy human oxygenated hemoglobin SERS spectra in the vicinity of 340cm-1 were weaker in patients with acute myeloid leukemia (2) The SERS peak of Raman peak at 655 cm-1 in SERS spectra of healthy volunteers was blue-shifted to around 670 cm-1 in the SERS spectrum of patients with acute myeloid leukemia and was around 727 cm-1 A new Raman peak appeared; (3) The relative intensities of Raman peaks at 472, 814, 1335, 1423 and 1588 cm-1 in oxyhemoglobin SERS spectra of healthy and acute leukemia patients were found to be relatively Intensity ratios I814 / I472, I1335 / I472, I1423 / I472 and I1588 / I472 can be used as a good diagnostic indicator of SERS in healthy people and patients with acute myeloid leukemia, which provides a preliminary diagnosis of acute myeloid leukemia based on SERS spectroscopy Experimental basis.