,Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal ne

• 作 者: Hong-sheng MIAO Lu-yang YU Guo-zhen HUI Li-he GUO
• 机 构: Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Aca
• 出 处: 中国药理学报（英文版）
• 发表日期: 2005年1期
• 关键词: A20 protein tumor necrosis factor primaryhippocampal neurons middle cerebral art

Aim: To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro. Methods: Primary hippocampal neurons in embryonic day 18 (El 8) rats were transfected with the A20 gene by using the new Nucleofector electroporation transfection method. We then examined, whether A20 -neurons possessed anti-apoptotic abilities after TNF-α stimulation in vitro.A20-neurons and pcDNA3 -neurons were transplanted into the penumbra of the brains of rats that had been subjected to 90-min of ischemia induced by left middle cerebral artery occlusion (MCAO). Results: A20-neurons resisted TNF-α induced apoptosis in vitro. The apoptosis rate of neurons overexpressing A20(28.46%±3.87%) was lower than that in neurons transfected with pcDNA3(53.06%±5.36%). More A20-neurons survived in the penumbra both 3-d and 7-d after transplantation than did sham pcDNA3 neurons. Conclusion: The novel function of A20 may make it a potential targets for the gene therapy for neurological diseases.