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根据已知植物抗病基因的序列以及蛋白激酶序列中的高保守区域设计合成了特异性和简并引物，用聚合酶链反应从水稻（ＯｒｙｚａｓａｔｉｖａＬ．）ＤＮＡ中扩增同源片段，获约１００个大小不同的克隆。以这些克隆作探针进行限制性片段长度多态性（ＲＦＬＰ）分析，已将２６个克隆定位在两个水稻分子标记连锁图１２条染色体的３４个位点上。其中１０个克隆与８个已定位的水稻抗病基因在分子标记连锁图上的位置对应或毗邻。用其中部分与抗稻瘟病基因在染色体位置相对应的克隆作探针，分析抗稻瘟病近等基因系，ＲＦＬＰ带型在抗性基因系和感病亲本间表现出多态性，表明这些克隆与抗病基因在染色体位置上有较好的对应关系。
Specific and degenerate primers were designed and synthesized based on the known plant disease resistance genes and the highly conserved regions in the protein kinase sequence. The homologous fragment was amplified from the DNA of Oryza sativa L. by polymerase chain reaction Clones of different sizes. Using these clones as probes for restriction fragment length polymorphism (RFLP) analysis, 26 clones have been mapped on 34 loci of 12 chromosomes in two rice molecular linkage maps. Among them, 10 clones corresponded to or were adjacent to the positions of the eight mapped rice resistance genes on the molecular linkage map. The RFLP bands of RFLP bands showed polymorphism between the resistant and susceptible parents using the clones with some of them corresponding to the chromosomal location of the blast resistance gene as a probe. The results showed that these clones And the resistance genes in the chromosome location has a good correspondence.