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目的探讨姜黄素对小鼠心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)的保护作用及其与Toll样受体4(Toll-like receptor 4,TLR4)/核因子-κB(nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)信号通路的相关性。方法取健康C57BL/6小鼠,采用随机区组法分为假手术组(Sham组)、MIRI组及姜黄素预处理的MIRI组(Cur组):使用丝线暂时阻断小鼠冠状动脉前降支(left anterior descending,LAD)血流30 min,再灌注4 h,建立小鼠MIRI模型;假手术组接受相同的手术过程,但未阻断LAD;Cur组在缺血前30 min腹腔注射姜黄素(100 mg/kg)。采用伊文思蓝和氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色评估小鼠心肌梗死面积;电化学发光法检测小鼠血清肌钙蛋白(cardiac troponin T,cTnT)水平;RT-PCR法检测小鼠心肌组织TLR4及肿瘤坏死因子-α(tumor nectosis factor-alpha,TNF-α,)mRNA水平;Western blot法检测小鼠心肌组织NF-κB蛋白表达水平;ELISA法检测小鼠心肌组织TNF-α蛋白表达水平。结果 Cur组小鼠心肌梗死面积及血清cTnT均低于MIRI组(P均<0.01);Cur组小鼠心肌组织TLR4、TNF-αm RNA水平及NF-κB、TNF-α蛋白表达水平均低于MIRI组(P均<0.05)。结论姜黄素对小鼠MIRI的心肌具有保护作用,可能是通过TLR4/NF-κB信号通路实现的。
Objective To investigate the protective effect of curcumin on myocardial ischemia-reperfusion injury (MIRI) and its relationship with Toll-like receptor 4 (TLR4) / nuclear factor-κB kappa-light-chain-enhancer of activated B cells, NF-κB) signaling pathway. Methods Healthy C57BL / 6 mice were randomly divided into sham operation group (Sham group), MIRI group and curcumin pretreatment MIRI group (Cur group): the use of silk thread to temporarily block the anterior descending coronary artery The left anterior descending (LAD) blood flow was performed for 30 min and reperfused for 4 h to establish the mouse MIRI model. The sham-operated group received the same surgical procedure but did not block the LAD. In the Cur group, Su (100 mg / kg). The areas of myocardial infarction in mice were assessed by Evans blue and TTC staining. The levels of cTnT, cTnT and cTnT ). The mRNA level of TLR4 and tumor necrosis factor-alpha (TNF-α) in myocardium were detected by RT-PCR. The protein expression of NF-κB in myocardium was detected by Western blot. Method was used to detect the expression of TNF-α in mouse myocardium. Results The myocardial infarct size and serum cTnT in Cur group were lower than those in MIRI group (all P <0.01). The levels of TLR4, TNF-αmRNA, NF-κB and TNF-α in Cur group were lower than those in MIRI group MIRI group (all P <0.05). Conclusion Curcumin has a protective effect on myocardium of MIRI mice, which may be through the TLR4 / NF-κB signaling pathway.