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目的 :探讨倒卵叶五加总皂甙 (SAOH)对大鼠心肌缺血再灌注后c fosmRNA表达的影响。方法 :采用结扎左冠状动脉前降支 30min ,再灌注复制大鼠心肌缺血再灌注损伤 (IRI)的模型 ,治疗组结扎前 10min注射SAOH ,检测心肌再灌注 4 0min时各组心肌组织乳酸脱氢酶 (LDH)、磷酸肌酸激酶 (CK)、丙二醛 (MDA)的含量和血浆超氧化物歧化酶 (SOD)的活性 ,并用原位杂交的方法观测左室心肌c fosmRNA的表达。结果 :再灌注 4 0min时 ,SAOH 5 0mg/kg组和 10 0mg/kg组心肌LDH、CK、MDA含量分别为 :(1790 .0± 31.4 )kIU/ g蛋白、(182 .0± 6 .8)kIU/ g蛋白、(3.6 9± 0 .11) μmol/ g蛋白和 (10 16 .0± 4 2 .6 )kIU/ g蛋白、(16 8.0± 4 .3)kIU/ g蛋白、(4.37± 0 .17) μmol/ g蛋白 ,显著低于IRI组 :(196 7.0± 6 4 .3)kIU/ g蛋白、(2 0 6 .0± 3.6 )kIU/ g蛋白、(5 .77±0 .2 0 ) μmol/ g蛋白 (均 P <0 .0 1) ;SAOH 5 0mg/kg组和 10 0mg/kg组SOD活性为 (11.70± 0 .2 6 ) μIU/L、(13.0 0± 0 .19) μIU/L ,较IRI组SOD(0 .30± 0 .2 5 ) μIU/L明显升高 (均 P <0 .0 1) ;而SAOH 5 0mg/kg组和 10 0mg/kg组心肌组织c fosmRNA的光密度值 (OD)分别为 0 .12± 0 .0 3、0 .12± 0 .0 3,较IRI组OD值 0 .2 1± 0 .0 3明显减小(均P
Objective : To investigate the effect of total saponin of Acanthopanax Obovatus (SAOH) on the expression of c fos mRNA after myocardial ischemia and reperfusion in rats. METHODS: The left anterior descending coronary artery was ligated for 30 min and reperfusion was performed to establish a rat model of myocardial ischemia-reperfusion injury (IRI). The SAOH was injected 10 min before the ligation of the treatment group. The contents of hydrogenase (LDH), phosphocreatine kinase (CK), malondialdehyde (MDA) and plasma superoxide dismutase (SOD) were measured. The expression of c fos mRNA in left ventricle myocardium was observed by in situ hybridization. RESULTS: At 40 min after reperfusion, the LDH, CK, and MDA levels in the SAOH 50 mg/kg and 100 mg/kg groups were (17900.0±31.4)kIU/g protein, (182.0±6.8). ) kIU/g protein, (3.6 9 ± 0 .11) μmol/g protein and (10 16 .0± 4 2 .6) kIU/g protein, (16 8.0 ± 4.3) kIU/g protein, (4.37 ± 0 .17) μmol / g protein, significantly lower than the IRI group: (196 7.0 ± 6 4 .3) kIU / g protein, (20 6 .0 ± 3.6) kIU / g protein, (5.77 ± 0 .2 0 ) μmol / g protein (all P <0.01); SOOH activity in the SAOH 50 mg/kg and 100 mg / kg groups was (11.70 ± 0.26) μIU/L, (13.0 0 ± 0). .19) μIU/L, significantly higher than IRI SOD (0.30 ± 0.25) μIU/L (all P <0.01); SAOH 50 mg/kg and 100 mg/kg The optical density (OD) of c fos mRNA in myocardial tissue was 0.12±0.30 and 0.12±0.03, respectively, which was significantly lower than that of IRI group (0.21±0.03). P