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为了解人疱疹病毒６型（ＨＨＶ－６）与鼻咽癌（ＮＰＣ）的可能关系，检测了ＮＰＣ发生不同阶段的石蜡组织中ＨＨＶ－６ＤＮＡ的存在情况。方法：采用ＰＣＲ、斑点杂交和原位杂交技术，共检测了４２例鼻咽癌石蜡组织、２１例鼻咽癌前病变石蜡组织和２７例正常鼻咽石蜡组织ＨＨＶ－６ＤＮＡ的存在情况。结果：通过ＰＣＲ扩增，在４２例ＮＰＣ组织中检出１３例存在ＨＨＶ－６ＤＮＡ，占３１％；２１例鼻咽癌前病变组织中１例阳性（４７％），而２７例正常鼻咽组织中无一例阳性。斑点杂交的结果表明ＰＣＲ扩增是ＨＨＶ－６特异的。原位杂交发现，在１３例ＰＣＲＨＨＶ－６阳性的ＮＰＣ组织中１１例ＨＨＶ－６ＤＮＡ阳性。ＨＨＶ－６ＤＮＡ主要分布于癌巢的肿瘤细胞核内，少数亦可见于癌旁的异型细胞，但在淋巴细胞及正常上皮细胞内未发现阳性信号。结论：本实验用分子生物学技术首次证明，在中国ＮＰＣ患者的鼻咽石蜡组织中存在ＨＨＶ－６。ＨＨＶ－６与ＮＰ相关，并有可能在ＮＰＣ的癌变过程中起一定的作用
To investigate the possible relationship between human herpesvirus type 6 (HHV-6) and nasopharyngeal carcinoma (NPC), the presence of HHV-6 DNA in paraffin tissues at different stages of NPC was examined. Methods: The presence of HHV-6 DNA was detected in 42 paraffin-embedded tissues of nasopharyngeal carcinoma, 21 paraffin-embedded tissues of nasopharyngeal precancerous lesions and 27 normal nasopharyngeal paraffin tissues by PCR, dot blot hybridization and in situ hybridization. Results: Thirteen cases of HHV-6 DNA were detected in 42 cases of NPC by PCR amplification, accounting for 31%. One case was positive in 21 cases of nasopharyngeal precancerous lesions (47%), while 27 cases of normal nasal No case of pharynx positive. The results of dot blotting indicated that PCR amplification is HHV-6 specific. In situ hybridization found that HHV-6 DNA was positive in 11 of 13 PCRHHV-6 positive NPC tissues. HHV-6DNA is mainly located in the tumor nucleus of cancer nests. A few of the HHV-6DNA can also be found in cancer-adjacent dysplastic cells, but no positive signal is found in lymphocytes and normal epithelial cells. CONCLUSIONS: This experiment demonstrated, for the first time, molecular biology techniques that HHV-6 is present in nasopharyngeal paraffin tissues of NPC patients in China. HHV-6 is associated with NP and may play a role in the carcinogenesis of NPC