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目的研究垂体腺苷酸环化酶激活肽（ＰＡＣＡＰ）对谷氨酸引起的海马神经元损伤的保护作用及其受体机制。方法海马神经元体外培养７ｄ，给予谷氨酸。结果当谷氨酸是０．１～１．０ｍｍｏｌ／Ｌ时，随着剂量的增加，神经元的存活率逐渐降低；１０－９ｍｏｌ／Ｌ～１０－１３ｍｏｌ／Ｌ的ＰＡＣＡＰ，能减轻谷氨酸引起的海马神经元损伤；ＰＡＣＡＰⅠ型受体特异性拮抗剂ＰＡＣＡＰ６－３８能抑制ＰＡＣＡＰ减轻谷氨酸对海马神经元损伤作用。结论ＰＡＣＡＰ具有减轻谷氨酸引起的海马神经元损伤的作用，该作用是由ＰＡＣＡＰⅠ型受体介导的。
Objective To study the protective effect of pituitary adenylate cyclase activating peptide (PACAP) on glutamate-induced damage of hippocampal neurons and its receptor mechanism. Methods Hippocampal neurons cultured in vitro 7d, given glutamate. Results When the glutamate concentration was 0.1-1.0 mmol / L, the survival rate of neurons decreased gradually with the increase of dose. PACAP of 10-9mol / L ~ 10-13mol / L reduced glutamate Induced damage of hippocampal neurons. PACAP6-38, a specific antagonist of PACAP type I, can inhibit the effect of PACAP on glutamate-induced neuronal damage in hippocampus. Conclusions PACAP has the effect of reducing glutamate-induced injury of hippocampal neurons, which is mediated by PACAP type Ⅰ receptor.