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目的通过观察氯氰菊酯(cypermethrin,CYP)对人乳腺癌细胞株MCF-7细胞增值的影响,探讨氯氰菊酯促进细胞增殖的生物学机制。方法 MCF-7细胞在加受试物前7 d改为无酚红完全培养基,以耗尽细胞内储存的雌激素。实验设为6组:无水乙醇(EtOH)溶剂对照组、雌二醇(E2)雌激素对照组、他莫西芬(TAM)抗雌激素对照组及氯氰菊酯3个浓度梯度(1.0×10-7mol/L、1.0×10-8mol/L、1.0×10-9mol/L)实验组。采用CCK8法检测各组细胞的增值情况,采用Hoechst 33342和Propidium Iodide双染荧光显微镜观察氯氰菊酯对雌激素耗尽诱导细胞凋亡作用影响。结果第4、5天细胞增殖实验,与EtOH溶剂对照组相比,除TAM抗雌激素组与EtOH溶剂对照组细胞增殖活力低外,其他4组细胞增殖活力低,依次为E2雌激素对照组、氯氰菊酯1.0×10-7mol/L组、氯氰菊酯1.0×10-8mol/L组、氯氰菊酯1.0×10-9mol/L组,P均<0.05;第3天与EtOH溶剂对照组比,E2雌激素对照组MCF-7细胞增殖活力高,P<0.05,第2天差异无统计学意义。荧光显微镜下见,EtOH溶剂对照组相比和TAM抗雌激素组细胞的凋亡作用明显,后者强于前者,而E2雌激素对照组、氯氰菊酯1.0×10-7mol/L组、氯氰菊酯1.0×10-8mol/L组、氯氰菊酯1.0×10-9mol/L组对细胞凋亡作用不明显。结论氯氰菊酯有可能是通过抑制乳腺癌细胞凋亡而发挥其促进细胞增殖作用的。
Objective To investigate the effect of cypermethrin (CYP) on the proliferation of human breast cancer cell line MCF-7 and to explore the biological mechanism of cypermethrin on cell proliferation. Methods MCF-7 cells were changed to phenol red-free complete medium 7 days before adding test substance to depletion of intracellular estrogen. The experiment was divided into 6 groups: ethanol (EtOH) solvent control group, estradiol (E2) estrogen control group, tamoxifen (TAM) antiestrogen control group and cypermethrin three concentration gradients (1.0 × 10- 7mol / L, 1.0 × 10-8mol / L, 1.0 × 10-9mol / L) experimental group. CCK8 assay was used to detect the proliferation of cells in each group. Hoechst 33342 and Propidium Iodide double staining fluorescence microscopy were used to observe the effects of cypermethrin on estrogen depletion-induced apoptosis. Results Compared with EtOH solvent control group, the cell proliferative activity in the 4th and 4th day was lower than that in the EtOH solvent control group except that the cell proliferative activity was low in the TAM antiestrogen group and the EtOH solvent control group, followed by E2 estrogen control group , Cypermethrin 1.0 × 10-7mol / L group, cypermethrin 1.0 × 10-8mol / L group, cypermethrin 1.0 × 10-9mol / L group, P <0.05; the third day compared with EtOH solvent control group, E2 estrogen control Group MCF-7 cells proliferation activity was high, P <0.05, the second day no significant difference. Under the fluorescence microscope, compared with the TAM anti-estrogen group, the EtOH solvent control group showed stronger apoptosis than the former, while E2 estrogen control group, cypermethrin 1.0 × 10-7mol / L group, cypermethrin 1.0 × 10-8mol / L group, cypermethrin 1.0 × 10-9mol / L group of apoptosis was not obvious. Conclusion Cypermethrin may play a role in promoting cell proliferation by inhibiting breast cancer cell apoptosis.