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目的研究骨碎补乙醇提取物对环磷酰胺诱导的免疫抑制小鼠细胞免疫功能的调节作用。方法 60只昆明种小鼠随机分为6组:空白对照组,低下模型组,阳性对照组(左旋咪唑0.05 g/kg),骨碎补醇提物低、中、高剂量组(2.5、5和10 g/kg),每组10只。4个给药组按10 m L/kg容积灌胃给药20 d,空白对照组和低下模型组给予等体积的生理盐水。除空白对照组外,其余5组小鼠于给药的第11~13天腹腔注射80 mg/kg环磷酰胺建立免疫抑制模型。实验结束后,检测小鼠脾淋巴细胞增殖能力,迟发型超敏反应,脾组织病理切片,外周血T淋巴细胞亚群CD_4~+、CD_8~+T细胞数,脾组织IFN-γ、IL-4 mRNA的表达等指标。结果 5 g/kg和10 g/kg骨碎补醇提物可以增强小鼠刀豆蛋白A诱导的脾脏T淋巴细胞增殖能力、迟发型超敏反应(DTH),提高外周血CD4+T细胞数、CD_4~+/CD_8~+比值、脾组织IFN-γmRNA的表达以及IFN-γ/IL-4比值;可以降低外周血CD_8~+T细胞数以及脾组织IL-4 mRNA的表达;但对脂多糖诱导的B淋巴细胞增殖反应无明显影响。结论骨碎补醇提物对环磷酰胺诱导的免疫抑制小鼠的细胞免疫功能有调节作用。
OBJECTIVE To study the regulatory effect of drynaria fortunei ethanol extract on cellular immune function induced by cyclophosphamide in immunosuppressed mice. Methods Sixty Kunming mice were randomly divided into 6 groups: blank control group, low model group, positive control group (levamisole 0.05 g / kg) And 10 g / kg), 10 in each. Four administration groups were administered intragastrically with a volume of 10 m L / kg for 20 days. The blank control group and the low model group were given an equal volume of normal saline. Except for the blank control group, the remaining 5 groups of mice were injected intraperitoneally with 80 mg / kg cyclophosphamide on the 11th to 13th day of administration to establish an immunosuppressive model. After the experiment, the spleen lymphocyte proliferative ability, delayed type hypersensitivity reaction, pathological section of spleen tissue, peripheral blood T lymphocyte subsets CD_4 ~ +, CD_8 ~ + T cells, spleen IFN-γ, IL- 4 mRNA expression and other indicators. Results The extracts of 5 g / kg and 10 g / kg Osteoporosis could enhance the ability of mouse concanavalin A to induce proliferation of T lymphocytes, delayed type hypersensitivity (DTH) and increase the number of CD4 + T cells in peripheral blood , CD_4 ~ + / CD_8 ~ + ratio, the expression of IFN-γmRNA and the ratio of IFN-γ / IL-4 in spleen tissue decreased the number of CD8 + T cells in peripheral blood and the expression of IL- Polysaccharide-induced B lymphocyte proliferation had no significant effect. Conclusion The extract of Rhizoma Drynariae can modulate the cellular immune function induced by cyclophosphamide in immunosuppressed mice.