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以2004年构建并保存在种质库10年的186个单株组成的湘743/Katy F2:3群体为材料,在发芽的第5天和第9天统计亲本和各株系的发芽率和成苗率,应用由129个标记组成的连锁图谱检测与种子活力相关的QTL,一共检测到12个QTLs,共分布在6条染色体的6个区间,单个QTLs对群体性状表型变异的贡献率为5.73%~47.53%,联合贡献率都是50%。其中,在第8染色体RM152~RM310区间检测到一个主效的QTL,对第5天发芽率和第9天发芽率和第9天成苗率的贡献率分别为12.02%、47.53%、38.64%,来自于湘743的基因增加发芽率和成苗率;在第9染色体RM444~RM219区间检测到一个稳定表达的QTL,对第5天发芽率和第9天发芽率和第9天成苗率的贡献率分别为8.85%、7.49%、10.36%,来自于Katy的基因增加发芽率和成苗率;此外,没有检测到显著的上位性互作。
A total of 186 individuals of Xiang 743 / Katy F2: 3, which were constructed in 2004 and kept in the germplasm bank for 10 years, were used as materials. The germination rate of the parents and each strain was calculated on the 5th and 9th day after germination The results showed that QTLs related to seed vigor were detected by linkage map consisting of 129 markers. A total of 12 QTLs were detected, which were distributed in 6 intervals of 6 chromosomes. The contribution rate of single QTLs to phenotypic variation of population traits 5.73% ~ 47.53%, the joint contribution rate is 50%. Among them, a major QTL was detected on RM152 ~ RM310 on chromosome 8, which contributed 12.02%, 47.53%, 38.64% respectively to the germination rate on the fifth day, the germination rate on the ninth day and the seedling rate on the ninth day, The gene from Xiang 743 increased the germination rate and seedling rate. A stable QTL was detected on the RM chromosome RM444 ~ RM219 on chromosome 9, which had the effect on the germination rate on the fifth day, the germination rate on the ninth day and the germination rate on the ninth day Rates were 8.85%, 7.49%, 10.36%, respectively. Genes from Katy increased germination and seedling rates; furthermore, no significant epistasis was detected.