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报道了喷多收中芸苔素内酯的测定方法,样品中加入适量的苯硼酸,用乙酸乙酯溶解,在80℃水浴上反应0.5h。于高效液相色谱紫外检测器[灵敏度:(AUFS)0.02]在波长222nm测定,该方法对芸苔素内酯在0.16mg/g—0.19mg/g水平上添加的回收率为97.09%—101.82%,平均变异系数为1.23%。
Reported the method of measuring the yield of brassinolide by adding appropriate amount of phenylboronic acid to the sample, dissolving it with ethyl acetate and reacting for 0.5h at 80 ℃ in water bath. In the high performance liquid chromatography UV detector [Sensitivity: (AUFS) 0.02] measured at a wavelength of 222nm, the method for the recovery of brassinolide 0.16mg / g-0.19mg / g level was 97.09% -101.82%, the average coefficient of variation was 1.23%.