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采用多聚酶链反应(PCR)测定白血病患者末梢血巨细胞病毒(HCMV)DNA,白血病组30例中22例阳性,对照组为30名健康献血员8例阳性,白血病组与对照组比较,P<0.01,差异非常显著,同时用ELISA测白血病组HCMV—IgM,16例阳性,对照组HCMV—IgM7例阳性。本文认为PCR能减少血清学方法的假阴性率,可以为严重的免疫机能低下的白血病患者筛选HCMV阴性的血制品,对于HCMV隐性感染者为极早期预防HCMV显性感染提供信息非常有益。
In the leukemia patients, peripheral blood cytomegalovirus (HCMV) DNA was detected by polymerase chain reaction (PCR) in 22 out of 30 cases of leukemia group, 30 healthy blood donors in the control group were positive in 8 cases, leukemia group compared with the control group, P < 0.01, the difference was very significant. Meanwhile, HCMV-IgM in leukemia group was detected by ELISA, positive in 16 cases and positive in HCMV-IgM in control group. This paper argues that PCR can reduce the false-negative rate of serological methods, screening HCMV-negative blood products for severe immunocompromised leukemia patients, and is very helpful for HCMV recessive infection to provide information for extremely early prevention of HCMV dominant infection.