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目的 应用原位杂交技术， 检测脑动脉瘤患者动脉瘤及其皮层小动脉标本中ＭＭＰ２ 、ＭＭＰ９、ＴＩＭＰ１、和ＴＩＭＰ２、的ｍＲＮＡ， 并定位产生它们的细胞。试图解释为什么脑动脉瘤的分布具有部位倾向性。方法 用地高辛标记的ＭＭＰ２、ＭＭＰ９、ＴＩＭＰ１、和ＴＩＭＰ２ ａｎｔｉｓｅｎｓｅ ｃＲＮＡ探针与脑动脉瘤及同一患者的皮层小动脉组织进行原位杂交， 定位产生它们的细胞。并用ｓｅｎｓｅ ｃＲＮＡ探针进行阴性对照。结果 所有脑动脉瘤标本中均见ＭＭＰ９ ｍＲＮＡ 阳性杂交信号。在内膜， 尤其是内弹力层部位， 阳性杂交信号密集存在。脑动脉瘤壁内弹力层消失。动脉瘤患者的皮层小动脉标本中未发现ＭＭＰ９ ｍＲＮＡ阳性杂交信号； 其内弹力层完整。在以上两种组织中均未发现ＭＭＰ２ 和ＴＩＭＰ１，２ ｍＲＮＡ的阳性杂交信号。结论 某些因素引起局部脑动脉的ＭＭＰ９ ｍＲＮＡ增多， 导致局部内弹力层断裂、消失， 甚至动脉壁全层结构的破坏， 从而进一步引发脑动脉瘤的形成， 而ＭＭＰ９ ｍＲＮＡ分布相对较少的部位无动脉瘤形成。
Objective To detect the mRNA of MMP-2, MMP-9, TIMP-1, and TIMP-2 in aneurysms and cortical arterioles of patients with cerebral aneurysms by in situ hybridization, and to locate their cells. Trying to explain why the distribution of cerebral aneurysms has site-like tendencies. Methods Digoxigenin-labeled MMP-2, MMP-9, TIMP-1, and TIMP-2 antisense cRNA probes were used to hybridize in situ with cerebral aneurysms and cortical arterioles of the same patient to locate their cells. A negative control was performed using the sense cRNA probe. Results MMP-9 mRNA positive hybridization signals were found in all cerebral aneurysm specimens. In the inner membrane, especially the inner elastic layer, positive hybridization signals are intensive. The elastic layer of the cerebral aneurysm disappeared. No MMP-9 mRNA positive hybridization signal was found in cortical arterioles of patients with aneurysm; its elastic layer was intact. No positive hybridization signals of MMP-2 and TIMP-1, 2 mRNA were found in the above two tissues. Conclusions Some factors cause the increase of MMP-9 mRNA in local cerebral arteries, resulting in the rupture and disappearance of local elastic layers, and even the destruction of the full-thickness structure of the arterial wall, which further triggers the formation of cerebral aneurysms. The distribution of MMP-9 mRNA is relatively low. There were no aneurysms in the few sites.