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目的探索肿瘤坏死因子-α(TNF-α)-308位点、白介素-1β(IL-1β)-511位点基因多态性与军团菌感染的关系。方法将32例军团菌感染者作为病例组,随机抽取100例军团菌检测阴性的肺部感染者为患者对照组,同期收集70例健康体检者为健康人对照组,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测3组TNF-α基因-308位点、IL-1β基因-511位点的单核苷酸多态性。结果病例组TNF-α-308基因型GG、GA、AA分别占75%、25%、0%,患者对照组GG、GA、AA基因型分别占87%、13%、0%,健康人对照组GG、GA、AA基因型分别占68.6%、31.4%、0%;病例组与患者对照组比较、病例组与健康人对照组比较,TNF-α-308位点基因型和等位基因频率差异无统计学意义(χ2=0.437、0.362,P=0.509、0.548;χ2=2.609、2.384,P=0.106、0.123);病例组IL-1β-511基因型CC、CT、TT分别占:0%、53.1%、46.9%,患者对照组CC、CT、TT基因型分别占3.4%、48.3%、48.3%,健康人对照组CC、CT、TT基因型分别占10%、51.4%、38.6%,3组比较,IL-1β-511位点基因型和等位基因频率差异无统计学意义(χ2=4.466,P=0.334;χ2=2.420,P=0.298)。结论 TNF-α-308位点、IL-1β-511位点的基因多态性与军团菌感染无关,TNF-α基因和IL-1β基因不是军团菌感染的易感基因。
Objective To explore the relationship between TNF-α-308 locus, interleukin-1β (IL-511) -511 polymorphism and Legionella infection. Methods A total of 32 cases of Legionella infection were selected as the case group and 100 cases of negative pulmonary infection were selected as the control group. In the same period, 70 cases of healthy volunteers were selected as healthy control group. Polymerase chain reaction- PCR-RFLP was used to detect SNP at -308 and IL-1β-511 in three groups of TNF-α gene. Results The GG, GA and AA genotypes of TNF-α-308 cases accounted for 75%, 25% and 0% respectively in the case group. The GG, GA and AA genotypes in the control group were 87%, 13% and 0% The genotypes of GG, GA and AA accounted for 68.6%, 31.4% and 0% respectively in the case group and the control group. The genotype and allele frequencies of TNF-α-308 locus in the case group were significantly higher than those in the control group There was no significant difference between the two groups (χ2 = 0.437,0.362, P = 0.509,0.548; χ2 = 2.609,2.384, P = 0.106,0.123) , 53.1% and 46.9% respectively. The CC, CT and TT genotypes in the control group were respectively 3.4%, 48.3% and 48.3% in the control group and 10%, 51.4% and 38.6% There was no significant difference in the genotype and allele frequencies of IL-1β-511 between the three groups (χ2 = 4.466, P = 0.334; χ2 = 2.420, P = 0.298). Conclusion The polymorphisms of TNF-α-308 locus and IL-1β-511 locus are not related to Legionella infection. The TNF-α gene and IL-1β gene are not the susceptible genes of Legionella infection.