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细胞色素P450氧化酶解毒代谢作用增强是棉铃虫Helicoverpa armigera对拟除虫菊酯类杀虫剂产生抗性的主要原因,棉铃虫细胞色素P450氧化酶基因CYP9A12的组成型过量表达与拟除虫菊酯抗性相关。为了进一步明确棉铃虫细胞色素P450氧化酶基因CYP9A12与拟除虫菊酯类杀虫剂抗性的关系,采用酿酒酵母Saccharomyces cerevisiae表达系统异源表达了CYP9A12基因,检测了该基因的酵母表达产物对溴氰菊酯、氟氯氰菊酯、甲氰菊酯和联苯菊酯4种药剂的离体代谢作用。结果表明:含有CYP9A12外源基因的重组酵母细胞裂解液对溴氰菊酯、氟氯氰菊酯和联苯菊酯的代谢率分别为8.58,5.85和3.94pmol/min·mg protein,而没有检测到对甲氰菊酯的代谢。本研究表明了CYP9A12具有代谢多种拟除虫菊酯的能力,也为CYP9A12参与拟除虫菊酯的解毒代谢提供了直接证据。
The enhancement of detoxification and metabolism of cytochrome P450 oxidase is the main reason for the resistance of the H. armigera Helicoverpa armigera to pyrethroid insecticides. The constitutive overexpression of the cytochrome P450 oxidase gene CYP9A12 of H. armigera was related to pyrethroid resistance . In order to further clarify the relationship between the cytochrome P450 oxidase gene CYP9A12 of Helicoverpa armigera and the resistance of pyrethroid insecticides, the CYP9A12 gene was heterologously expressed using Saccharomyces cerevisiae expression system and the expression of the CYP9A12 gene In vitro metabolism of four agents, pyrethroids, cyfluthrin, fenpropathrin and bifenthrin. The results showed that the metabolic rates of deltamethrin, cyfluthrin and bifenthrin in recombinant yeast cell lysate containing CYP9A12 exogenous gene were 8.58, 5.85 and 3.94 pmol / min · mg protein respectively, Metabolism of cypermethrin. This study shows that CYP9A12 has the ability to metabolize many pyrethroids and provides direct evidence that CYP9A12 is involved in the detoxification of pyrethroids.