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目的 :研究肺癌患者外周血单个核细胞 (PBMCs)分泌 IL- 1、IL- 4、IL- 6的水平 ,及根治手术对其影响。方法 :分别采用生物学方法及酶联免疫方法测定 40例肺癌手术前、14例肺癌根治手术前后、30例正常对照者及 2 2例肺部良性疾病者细胞因子的水平。结果 :肺癌组分泌 IL- 1、IL- 4、IL- 6 (分别为 0 .5 6 6± 0 .10 2 ,7.918± 5 .0 71pg/ ml,3.499± 2 .0 88)均低于正常对照组 (分别为 0 .6 40± 0 .12 3,19.2 97± 10 .6 85 pg/ ml,5 .372± 0 .113,均 P <0 .0 1) ,且相互间无相关性。根治手术后产生 IL- 1、IL- 4水平明显提高 (分别为 t=5 .0 6 7,P <0 .0 1;t=2 .2 44 ,P <0 .0 5 )。结论 :检测 PBMCs分泌 IL- 1、IL- 4水平对肺癌的诊断有较好的敏感性和特异性 ,联合检测意义更大 ,且对监测疗效 ,提示预后也有重要的临床意义。
Objective: To study the levels of IL-1, IL-4 and IL-6 secreted by peripheral blood mononuclear cells (PBMCs) in patients with lung cancer, and the effect of radical surgery on them. METHODS: The cytokine levels of 40 patients with lung cancer, 14 patients with lung cancer before and after surgery, 30 normal controls and 22 patients with benign lung disease were measured by biological methods and enzyme-linked immunosorbent assay, respectively. Results: The levels of IL-1, IL-4 and IL-6 secreted by lung cancer group were lower than normal (0.66±0.102, 7.918±5.01 pg/ml and 3.499±2.08, respectively). The control group (0.64 ± 0.123, 19.27 97 ± 10.685 pg/ml, 5.372 ± 0.113, respectively, all P <0.01) had no correlation with each other. After radical surgery, IL-1 and IL-4 levels increased significantly (t = 5.06, P < 0.01; t = 2.24, P <0.05). Conclusion : The detection of PBMCs secreting IL-1 and IL-4 levels has good sensitivity and specificity in the diagnosis of lung cancer. The combined detection has greater significance, and it also has important clinical significance for monitoring the curative effect.