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应用铜－镉还原微盘测定法、硝酸镧示踪法及电镜技术初步观察研究小鼠Ｈ２２腹水型肝癌接种后不同时期血清ＮＯ水平的变化及肿瘤细胞在腹腔内大量增殖、生长对宿主肠壁组织细胞通透性及超微结构的影响。实验结果显示，与对照组相比，接种早期（３天），血清ＮＯ水平明显上升，以后逐渐下降，晚期时（第１０，１３天）明显低于对照组。早期时即可见镧颗粒进入细胞内，进入中期后，肠壁组织超微结构发生明显损伤，并可见细胞坏死。提示早期血清ＮＯ水平的增高可能为外源性肿瘤细胞刺激机体免疫系统所至，而晚期ＮＯ水平下降则可能与机体代谢功能紊乱，免疫功能下降有关。膜通透性的改变早于超微结构的改变。
Using copper-cadmium reduction microplate assay, lanthanum nitrate tracer method and electron microscopy to observe the changes of serum NO levels at different periods after inoculation of mouse H22 ascitic liver cancer and the proliferation and growth of tumor cells in the abdominal cavity of the host. Cellular permeability and ultrastructure effects. The experimental results showed that compared with the control group, the serum NO level increased significantly in the early stage of inoculation (3 days), and then gradually decreased. The late stage (10th and 13th days) was significantly lower than the control group. In the early stage, the granules can be seen entering into the cell. After entering the middle stage, the ultrastructure of the intestinal wall tissue is obviously damaged, and cell necrosis can be seen. It is suggested that the increase of NO level in early serum may be caused by exogenous tumor cells stimulating the body’s immune system, and the decline of late NO level may be related to metabolic dysfunction and decreased immune function. Changes in membrane permeability precede changes in ultrastructure.