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目的应用ADCF无血清细胞培养液对分泌鼠疫F1McAb的杂交瘤细胞进行静止培养,初步了解该瘤株在ADCF中的生长情况,为今后采用无血清培养液体外培养法生产单克隆抗体提供依据。方法通过将杂交瘤细胞直接转入ADCF培养液培养、驯化培养和优化条件后培养,观察细胞在各种培养条件下的生长情况,进行活细胞计数和抗体效价检测。结果用ADCF培养鼠疫F1杂交瘤细胞,细胞直接转入不能较好的生长;通过驯化、在放过含血清培养液的原瓶中能较好的贴壁生长,在新瓶中不能稳定地贴壁生长和增殖;在经含血清的培养液优化后的培养瓶中,细胞能贴壁生长和增殖。结论ADCF培养液可用于分泌鼠疫F1McAb的杂交瘤细胞的培养。
Objective To study the growth of hybridoma cells secreting plague F1McAb by using ADCF serum-free cell culture medium to understand the growth of this strain in ADCF and to provide a basis for the future production of monoclonal antibodies by serum-free culture medium. Methods The hybridoma cells were directly transferred into ADCF medium for culture, domestication and culture. The growth of cells was observed under various culture conditions, and the viable cell count and antibody titer were measured. Results With the use of ADCF, the F1 hybridoma cells were cultured in vitro, and the cells could not grow well when they were directly transferred into the cells. After acclimation, Wall growth and proliferation; cells were adherently grown and proliferated in culture flasks optimized with serum-containing media. Conclusion ADCF culture medium can be used for the culture of hybridoma cells secreting F1McAb.