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Evaluation on the impact of heavy ions on the physiological and intellective aspects of young patients after heavy ion therapy is of extreme importance for optimizing the treatment plans. For this purpose, young mice were irradiated with relatively low LET carbon ions at the plateau region at low dose in this work. Twenty-one-day-old female C57BL/J6 mice, which are roughly equivalent to toddler children, were used in our experiment. 16 mice were randomly divided into two groups and one group of mice was exposed to relatively low LET (15 keV/μm, lower than that around the Bragg peak region) carbon ions at 1 Gy under the condition of whole-body ion le traversal. After irradiation, food-intake and body weight of the mice were recorded at every morning for three weeks. 21 d after irradiation, the capacity of learning and memory was tested for all the mice with Morris water maze. The main component of the water maze set-up is a round pool of about 1.6 m in diameter and about 0.5 m in depth. The tasks were recorded with a video camera. All sides of the maze were within the camera’s field of view. The water maze was filled with tap water (temperature about 19 ℃). An escape platform was just below the surface of the water, and not visible because the water was made opaque with milk. All the animals were pre-trained for the water maze experiment.
Evaluation on the impact of heavy ions on the physiological and intellective aspects of young patients after heavy ion therapy is of extreme importance for optimizing the treatment plans. For this purpose, young mice were irradiated with relatively low LET carbon ions at the plateau region at low dose in this work. Twenty-one-day-old female C57BL / J6 mice, which are roughly equal to toddler children, were used in our experiment. 16 mice were randomly divided into two groups and one group of mice was exposed to relatively low LET (15 keV / μm, lower than that around the Bragg peak region) carbon ions at 1 Gy under the condition of whole-body ion le traversal. After irradiation, food-intake and body weight of the mice were recorded at every morning for three weeks. 21 d after irradiation, the capacity of learning and memory was tested for all the mice with Morris water maze. The main component of the water maze set-up is a round pool of about 1.6 m in diameter and about 0.5 m in depth The tasks were recorded with a video camera. All sides of the maze were within the camera’s field of view. The water maze was filled with tap water (temperature about 19 ° C). An escape platform was just below the surface of the water, and not visible because the water was made opaque with milk. All the animals were pre-trained for the water maze experiment.