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目的 研究丹参水溶性化合物--丹酚酸A、丹酚酸B及原儿茶醛对Cr(Ⅵ)诱导的蚕豆根尖细胞遗传毒性的影响.方法 通过蚕豆根尖微核试验与染色体畸变试验,观察5～1000 μg/ml浓度范围的丹酚酸A、丹酚酸B、原儿茶醛单独及与100 μg/ml Cr(Ⅵ)联合染毒对蚕豆根尖细胞MNR、MI、CAR的影响.对照组采用蒸馏水.结果 与对照组比较,50、100μg/ml丹酚酸A单独染毒组,50μg/ml丹酚酸B单独染毒组.100、500 μg/ml原儿茶醛单独染毒组蚕豆根尖细胞M1较高,差异有统计学意义(P＜0.05或P＜0.01).不同剂量丹酚酸A、丹酚酸B、原儿茶醛单独染毒组蚕豆根尖细胞MNR、CAR与对照组相比,差异无统计学意义(P＜0.05).与对照组比较,5 μg/ml丹酚酸A+100μg/nd Cr(Ⅵ)染毒组、5 μg/ml丹酚酸B+100 μg/ml Cr(Ⅵ)染毒组、5、10 μg/ml原儿茶醛+100 μg/ml Cr(Ⅵ)染毒组蚕豆根尖细胞MNR以及1000 μg/ml原儿茶醛+100 μg/ml Cr(Ⅵ)染毒组蚕豆根尖细胞M1较高,差异有统计学意义(P＜0.05或P＜0.01).不同剂量丹酚酸A、丹酚酸B、原儿茶醛与100μg/ml cr(Ⅵ)联合染毒组蚕豆根尖细胞CAR间比较,差异均无统计学意义(P＞0.05).与Cr(Ⅵ)染毒组比较,各剂量(5-1000μg/ml)丹酚酸A、丹酚酸B、原儿茶醛与Cr(Ⅵ)联合染毒组蚕豆根尖细胞MNR、CAR较低,10、100、500、1 000 μg/ml丹酚酸A+100 μg/ml Cr(Ⅵ)染毒组,5、50 μg/ml丹酚酸B+100 μg/ml Cr(Ⅵ)染毒组和1000 μg/ml原儿茶醛+100μg/ml Cr(Ⅵ)染毒组蚕豆根尖细胞M1较高,差异均有统计学意义(P＜0.05或P＜0.01).结论 丹酚酸A、丹酚酸B、原儿茶醛能够抑制Cr(Ⅵ)诱导的蚕豆根尖细遗传毒性,修复细胞损伤并调节细胞分裂生长.“,”Objective To study the antagonism of water-soluble compounds of salvia-salvianolie acid A(SalA), salvianolic acid B(SalB) and catechu aldehyde(CA) to Cr (Ⅵ) induced genetic toxicity in Viciafaba root tip cells. Methods The root tip of Vicia faba were treated with SalA, SalB, CA(5-1000 μg/ml) and every compound combined with 100 μg/ml Cr(Ⅵ) respectively, and with distilled water as control group. The micronucleus rate (MNR), the mitotic index (MI) and the chromosome aberration rate(CAR) of Viciafaba root tip cells were observed by micronucleus assay and chromosome aberration assay. Results Compared with controlgroup, treatments of 50,100 μg/ml SalA, 50 μg/ml SalB, 100,500 μg/ml CA significantly increased MI (P＜0.05 or P＜0.01). There were no obvious differences of the MNR and CAR in SalA treated group, SalB treated group, CA treated group compared with the control group(P＜0.05).There were remarkable increase of the MNR in the treatments of 5 μg/ml SalA+lO0 μg/ml Cr(Ⅵ), 5 μg/ml SalB+100 μg/ml Cr(Ⅵ), 5,10 μg/ml CA +100 μg/ml Cr(Ⅵ) and the MI in the treatments of 1000 μg/ml CA +100 μg/ml Cr(Ⅵ)compared with the control group(P＜0.05 or P＜0.01).There were no obvious differences of the CAR in SalA, SalB, CA combined with 100 μg/ml Cr(Ⅵ) treated group compared with control group(P＜0.05).Compared with Cr(Ⅵ) treated group, treatments of SalA+100 μg/ml Cr(Ⅵ),SalB+100 μg/ml Cr(Ⅵ),CA+100 μg/ml Cr(Ⅵ)(5-1000 μg/ml) showed a significant decrease on the MNR,CAR (P＜0.05 or P＜0.01) and treatments of 10,100,500 and 1000 μg/ml SalA+100 μg/ml Cr(Ⅵ), 5, 50 μg/ml SalB+100 μg/ml Cr(Ⅵ),100μg/ml CA+100 μg/ml Cr(Ⅵ) significantly increased the level of MNR (P＜0.05 or P＜0.01). Conclusion SalA, SalB and CA have an obvious antagonism to Cr (Ⅵ) induced genetic toxicity in Viciafaba root tip cells.