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目的 研究亚低温对缺氧缺血性脑损伤的保护作用 ,探讨亚低温治疗的最佳时机、深度、持续时间。方法 Wistar 7日龄新生大鼠 ,随机分为 :1) 2 9℃低温干预组、34℃低温干预组、常温 (37℃ )恢复组、正常对照组 (假手术组 ) ;2 ) 2 4h低温干预组、4 8h低温干预组、72h低温干预组、常温 (37℃ )恢复组、正常对照组 (假手术组 ) ;3)缺氧缺血后 0min起始低温干预组、6 0min起始低温干预组、12 0min起始低温干预组、6h起始低温干预组、常温 (37℃ )恢复组、正常对照组 (假手术组 )。每组均 2 0只大鼠均于缺氧缺血后 78h处死 ,测定脑组织含水量及微管相关蛋白 2 (MAP 2 )免疫组织化学染色 ,计算脑损伤程度。结果 1) 2 9℃低温干预效果优于 34℃低温干预效果。 2 ) 72h低温干预效果优于 2 4、4 8h低温干预。 3)缺氧缺血后立即低温干预效果好 ,延迟 6h的低温干预仍有效。结论 缺氧缺血后立即 2 9℃低温干预 72h有显著脑保护作用
Objective To study the protective effect of mild hypothermia on hypoxic-ischemic brain damage and to explore the optimal timing, depth and duration of mild hypothermia therapy. Methods Wistar 7-day-old neonatal rats were randomly divided into 1) 29 ℃ low temperature intervention group, 34 ℃ low temperature intervention group, normal temperature (37 ℃) recovery group, normal control group (sham operation group); 2) Low temperature intervention group, 48h hypothermia intervention group, 72h hypothermia intervention group, normal temperature (37 ℃) recovery group and normal control group (sham operation group); 3) hypothermic ischemia group 0min initial low temperature intervention group, 60min initial hypothermia In the intervention group, the initial low temperature intervention group at 120 minutes, the initial low temperature intervention group at 6 hours, the normal temperature (37 ° C) recovery group and the normal control group (sham operation group). Twenty rats in each group were sacrificed at 78 hours after hypoxia-ischemia. The water content of brain tissue and the immunohistochemical staining of MAP2 were measured and the degree of brain injury was calculated. Results 1) 29 ℃ low temperature intervention effect is better than 34 ℃ low temperature intervention effect. 2) 72h low temperature intervention better than 2 448h low temperature intervention. 3) Hypothermia immediately after hypoxic-ischemic intervention is effective, low-temperature delayed 6h intervention is still valid. Conclusion Hypothermia immediately after 29 h hypothermia intervention 72h significant cerebral protection