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从饲料中分离类圆线虫幼虫方法为:将样本漂洗2次,漂洗液以Smeal和Hendg法沉淀浓缩到200ml左右,移至1kg抽气瓶中抽气使呈23mmHg柱的半真空状态。悬浮液置于250ml量简内静止过夜。弃上清液至沉淀样本体积与总体积之比小于3:5。量筒
The method of isolating Rhodospirillum larvae from feed was as follows: the sample was rinsed twice, and the rinsing solution was concentrated to about 200 ml by Smeal and Hendg method, transferred to a 1-kg extraction flask and evacuated to give a semi-vacuum state of 23 mm Hg column. The suspension was placed in a volume of 250ml Jane overnight. The ratio of the supernatant to sediment sample volume to total volume is less than 3: 5. Measuring cylinder